Quinolone-induced upregulation of osteopontin gene promoter activity in human lung epithelial cell line A549

Beata Shiratori, Jing Zhang, Osamu Usami, Haorile Chagan-Yasutan, Yasuhiko Suzuki, Chie Nakajima, Toshimitsu Uede, Toshio Hattori

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Quinolones, in addition to their antibacterial activities, act as immunomodulators. Osteopontin (OPN), a member of the extracellular matrix proteins, was found to play a role in the immune and inflammatory response. We found that quinolones significantly enhanced OPN secretion, namely, garenoxacin (220%), moxifloxacin (62%), gatifloxacin (82%), sparfloxacin, (79%), and sitafloxacin (60%). Enhancement of OPN secretion was shown to be due to the effect of quinolones on the OPN gene promoter activity. We also examined the role of quinolones on apoptosis and found that sparfloxacin decreased the late apoptosis of A549 cells, but garenoxacin did not show the antiapoptotic effect. The antiapoptotic effects of quinolones do not appear to be associated with OPN elevation.

Original languageEnglish
Pages (from-to)2868-2872
Number of pages5
JournalAntimicrobial agents and chemotherapy
Volume56
Issue number6
DOIs
Publication statusPublished - 2012 Jun

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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    Shiratori, B., Zhang, J., Usami, O., Chagan-Yasutan, H., Suzuki, Y., Nakajima, C., Uede, T., & Hattori, T. (2012). Quinolone-induced upregulation of osteopontin gene promoter activity in human lung epithelial cell line A549. Antimicrobial agents and chemotherapy, 56(6), 2868-2872. https://doi.org/10.1128/AAC.06062-11