Quantitative trait loci affecting Rous Sarcoma Virus induced tumor regression trait in F2 intercross chickens

Y. Uemoto, J. Saburi, S. Sato, S. Odawara, T. Ohtake, R. Yamamoto, T. Miyata, K. Suzuki, H. Yamashita, C. Irina, G. Plastow, T. Mitsuhashi, E. Kobayashi

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

We performed a genome-wide linkage and quantitative trait locus (QTL) analysis to confirm the existence of QTL affecting Rous Sarcoma Virus (RSV) induced tumor regression, and to estimate their effects on phenotypic variance in an F2 resource population. The F2 population comprised 158 chickens obtained by crossing tumor regressive White Leghorn (WL) and tumor progressive Rhode Island Red (RIR) lines was measured for tumor formation after RSV inoculation. Forty-three tumor progressive and 28 tumor regressive chickens were then used for genome-wide linkage and QTL analysis using a total of 186 microsatellite markers. Microsatellite markers were mapped on 20 autosomal chromosomes. A significant QTL was detected with marker LEI0258 located within the MHC B region on chromosome 16. This QTL had the highest F ratio (9.8) and accounted for 20.1% of the phenotypic variation. Suggestive QTL were also detected on chromosomes 4, 7 and 10. The QTL on chromosome 4 were detected at the 1% chromosome-wide level explaining 17.5% of the phenotypic variation, and the QTLs on chromosome 7 and 10 were detected at the 5% chromosome-wide level and explained 11.1% and 10.5% of the phenotypic variation, respectively. These results indicate that the QTLs. in the non-MHC regions play a significant role in RSV-induced tumor regression. The present study constitutes one of the first preliminary reports in domestic chickens for QTLs affecting RSV-induced tumor regression outside the MHC region.

Original languageEnglish
Pages (from-to)1359-1365
Number of pages7
JournalAsian-Australasian Journal of Animal Sciences
Volume22
Issue number10
DOIs
Publication statusPublished - 2009 Oct

Keywords

  • Chicken
  • Non-MHC
  • Quantitative Trait Locus
  • Subgroup A Rous Sarcoma Virus
  • Tumor Regression

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Engineering(all)

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