Quantitative PET analysis of the dopamine D2 receptor agonist radioligand 11C-(R)-2-CH3O-N-n-propylnorapomorphine in the human brain

Tatsui Otsuka, Hiroshi Ito, Christer Halldin, Hidehiko Takahashi, Harumasa Takano, Ryosuke Arakawa, Masaki Okumura, Fumitoshi Kodaka, Michie Miyoshi, Mizuho Sekine, Chie Seki, Ryuji Nakao, Kazutoshi Suzuki, Sjoerd Finnema, Yoshio Hirayasu, Tetsuya Suhara, Lars Farde

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

It has been demonstrated in vitro that the dopamine D2 receptor has 2 interconvertible affinity states for endogenous dopamine, referred to as the high- and the low-affinity states. 11C-(R)-2-CH 3O-N-n-propylnorapomorphine (11C-MNPA) is a new agonist radioligand for in vivo imaging of the high-affinity state of dopamine D 2 receptors using PET. In the present study, the kinetics of 11C-MNPA were examined for the first time, to our knowledge, in the human brain and analyzed using quantitative approaches with or without an arterial input function. Methods: A 90-min dynamic PET scan was obtained for 10 healthy men after an intravenous injection of 11C-MNPA. The binding potential (BPND) was calculated using the indirect kinetic method, a kinetic compartment analysis with a metabolite-corrected arterial input function. BPND was also calculated by the simplified reference tissue model (SRTM) and transient equilibrium methods, both with the cerebellum as the reference brain region. The results of the quantitative methods were compared in a cross-validation approach. Results: The highest regional radioactivity was observed in the putamen. BPND values obtained by kinetic analysis were 0.82 6 0.09, 0.59 6 0.11, and 0.28 6 0.06, respectively, in the putamen, caudate, and thalamus. BPND values obtained by the SRTM and transient equilibrium methods were in good agreement with those obtained by the indirect kinetic method (r = 0.98 and r = 0.93, respectively). For all quantification methods, the BPND values based on data acquired from 0 to 60 min were in good agreement with those based on data acquired from 0 to 90 min (r = 0.90-0.99). Conclusion: The regional distribution of 11C-MNPA binding was in good agreement with previous PET studies of dopamine D2 receptors in the human brain using antagonist radioligands. The results support routine use of the SRTM and transient equilibrium methods, that is, methods that do not require an arterial input function and need a scan time of only about 60 min. 11C-MNPA should thus be useful for clinical research on the pathophysiology of neuropsychiatric disorders and estimation of dopamine D 2 receptor occupancy by dopaminergic drugs.

Original languageEnglish
Pages (from-to)703-710
Number of pages8
JournalJournal of Nuclear Medicine
Volume50
Issue number5
DOIs
Publication statusPublished - 2009 May 1
Externally publishedYes

Keywords

  • Agonist
  • C-MNPA
  • Dopamine D receptor
  • Human
  • Positron emission tomography

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'Quantitative PET analysis of the dopamine D<sub>2</sub> receptor agonist radioligand <sup>11</sup>C-(R)-2-CH<sub>3</sub>O-N-n-propylnorapomorphine in the human brain'. Together they form a unique fingerprint.

Cite this