Quantitative PCR determination of human cytomegalovirus in blood cells

J. Satou, T. Funato, N. Satoh, Y. Abe, K. K. Ishii, T. Sasaki, M. Kaku

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

We evaluated a rapid and sensitive method to determine human cytomegalovirus (CMV) DNA levels in blood cells using a quantitative polymerase chain reaction (PCR) technique. This method is based on real-time detection of PCR using a dual fluorescence-labeled probe and a sequence detector. Ten copies of CMV DNA were detected, when 1 μg of DNA from blood samples was used with this method, and a good correlation was obtained between increased concentrations of copy numbers calculated and measured copy numbers of CMV DNA (r = 0.999). Forty normal subjects exhibited no copies of CMV DNA. On the other hand, a 6-month-old girl tested positive for increased levels 4 weeks after liver transplant. This method is simple, accurate, and sensitive for the quantitative detection of CMV DNA in vivo, indicating possible applications for the diagnosis and monitoring of CMV infection.

Original languageEnglish
Pages (from-to)122-126
Number of pages5
JournalJournal of Clinical Laboratory Analysis
Volume15
Issue number3
DOIs
Publication statusPublished - 2001

Keywords

  • Cytomegalovirus (CMV)
  • Liver transplantation
  • Polymerase chain reaction (PCR)
  • Quantitative PCR

ASJC Scopus subject areas

  • Immunology and Allergy
  • Hematology
  • Public Health, Environmental and Occupational Health
  • Clinical Biochemistry
  • Medical Laboratory Technology
  • Biochemistry, medical
  • Microbiology (medical)

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