Quantitative measurement of spliced XBP1 mRNA as an indicator of endoplasmic reticulum stress

Morihiko Hirota, Masato Kitagaki, Hiroshi Itagaki, Setsuya Aiba

Research output: Contribution to journalArticlepeer-review

82 Citations (Scopus)

Abstract

The unfolded protein response (UPR) events triggered by the accumulation of unfolded protein in endoplasmic reticulum (ER) activate the three UPR signaling pathways mediated by IRE1, ATF6 and PERK. Spliced XBP1 mRNA induced by activated IRE1 is translated to the protein, a potent transcription factor that induces BiP expression. XBP1 is also induced by activated ATF6. It is thus thought to be an important marker reflecting both IRE1 and ATF6 signaling in response to ER stress. For quantitative measurement of XBP1 gene expression, it is important to distinguish between the spliced and non-spliced form of XBP1 mRNA. We have developed a new method to detect the spliced XBP1 mRNA by means of real-time PCR and we compared the result with measurements of the expression of the ER stress inducible gene BiP. A good correlation was found between spliced XBP1 expression and BiP expression. Thus, our method may be useful for simple and quantitative evaluation of ER stress.

Original languageEnglish
Pages (from-to)149-156
Number of pages8
JournalJournal of Toxicological Sciences
Volume31
Issue number2
DOIs
Publication statusPublished - 2006

Keywords

  • BiP
  • ER stress
  • Quantitative measurement
  • Spliced XBP1
  • THP-1

ASJC Scopus subject areas

  • Toxicology

Fingerprint Dive into the research topics of 'Quantitative measurement of spliced XBP1 mRNA as an indicator of endoplasmic reticulum stress'. Together they form a unique fingerprint.

Cite this