Quantitative HPLC analysis of acidic opines by phenylthiocarbamyl derivatization

M. Sato, S. Suzuki, Y. Yasuda, H. Kawauchi, N. Kanno, Y. Sato

    Research output: Contribution to journalArticlepeer-review

    23 Citations (Scopus)

    Abstract

    A new method for the quantitative analysis of acidic opines-alanopine, strombine, tauropine, and β-alanopine-is presented. The method is based on formation of phenylthiocarbamyl (PTC) derivatives of the acidic opines after partial purification by ion-exchange chromatography. The PTC acidic opines are separated by reverse-phase high-performance liquid chromatography (HPLC) and detected within 20 min by ultraviolet absorbance. This HPLC method gives higher sensitivity, 10-30 pmol minimum detection, and better reproducibility than the high-voltage paper electrophoresis method. There is also good agreement for the three acidic opines (alanopine, strombine, and tauropine) when compared by HPLC and electrophoresis methods. Accumulation of β-alanopine was observed for the first time in the adductor muscle of blood shell, Scapharca broughtonii, during aerial exposure by application of the HPLC detection method.

    Original languageEnglish
    Pages (from-to)623-627
    Number of pages5
    JournalAnalytical Biochemistry
    Volume174
    Issue number2
    DOIs
    Publication statusPublished - 1988 Nov 1

    Keywords

    • HPLC determination
    • Mollusca
    • imino acids
    • metabolites
    • opines
    • phenylthiocarbamyl derivative

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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