Quantitative HPLC analysis of acidic opines by phenylthiocarbamyl derivatization

Minoru Sato, S. Suzuki, Y. Yasuda, H. Kawauchi, N. Kanno, Y. Sato

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A new method for the quantitative analysis of acidic opines-alanopine, strombine, tauropine, and β-alanopine-is presented. The method is based on formation of phenylthiocarbamyl (PTC) derivatives of the acidic opines after partial purification by ion-exchange chromatography. The PTC acidic opines are separated by reverse-phase high-performance liquid chromatography (HPLC) and detected within 20 min by ultraviolet absorbance. This HPLC method gives higher sensitivity, 10-30 pmol minimum detection, and better reproducibility than the high-voltage paper electrophoresis method. There is also good agreement for the three acidic opines (alanopine, strombine, and tauropine) when compared by HPLC and electrophoresis methods. Accumulation of β-alanopine was observed for the first time in the adductor muscle of blood shell, Scapharca broughtonii, during aerial exposure by application of the HPLC detection method.

Original languageEnglish
Pages (from-to)623-627
Number of pages5
JournalAnalytical Biochemistry
Volume174
Issue number2
DOIs
Publication statusPublished - 1988 Nov 1

Keywords

  • HPLC determination
  • Mollusca
  • imino acids
  • metabolites
  • opines
  • phenylthiocarbamyl derivative

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Sato, M., Suzuki, S., Yasuda, Y., Kawauchi, H., Kanno, N., & Sato, Y. (1988). Quantitative HPLC analysis of acidic opines by phenylthiocarbamyl derivatization. Analytical Biochemistry, 174(2), 623-627. https://doi.org/10.1016/0003-2697(88)90065-6