Quantitative analysis of thyroid-stimulating hormone messenger RNA and heterogeneous nuclear RNA in hypothyroid rats

Koreaki Sugimoto, Kouki Mori, Katsuya Uchida, Daisuke Kobayashi, Keiichi Itoi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Thyroid-stimulating hormone (TSH) stimulates the synthesis and release of thyroid hormones including triiodothyronine (T3) and thyroxine (T4). Semiquantitative analyses using northern blot and in situ hybridization suggested that TSH gene transcription is upregulated under conditions of hypothyroidism. However, no quantitative analysis of TSH gene expression using real-time polymerase chain reaction (PCR) has been reported. In this study, we quantitated the TSHβ messenger ribonucleic acid (mRNA) level as well as the TSHβ heterogeneous nuclear ribonucleic acid (hnRNA) level in the anterior pituitary of hypothyroid rats, by real-time PCR using the LightCycler® system. The hnRNA is the primary deoxyribonucleic acid (DNA) transcript, which reflects the transcription rate more reliably than the mRNA because of its short half-life. In the anterior pituitary of rats with methimazol-induced chronic hypothyroidism, both mRNA and hnRNA expression of TSHβ were upregulated fourfold relative to normal rats (n = 4). Our method provides a rapid and accurate measure of gene transcription. In the present report, we described a technique for accurate measurement of TSHβ hnRNA level.

Original languageEnglish
Pages (from-to)142-146
Number of pages5
JournalBrain Research Bulletin
Volume74
Issue number1-3
DOIs
Publication statusPublished - 2007 Sep 14

Keywords

  • Heterogeneous nuclear RNA (hnRNA)
  • LightCycler
  • Real-time PCR
  • Thyroid-stimulating hormone (TSH
  • Thyrotropin-releasing hormone (TRH)
  • thyrotropin)

ASJC Scopus subject areas

  • Neuroscience(all)

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