Quantitative analysis of subunit mismatch arrangement in staphylococcal gamma-hemolysin heteroheptameric transmembrane pore

Noriko Tomita, Kazuyo Abe, Makoto Ohta

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

Pore-forming cytolytic proteins distributed in a wide variety of eukaryotic and prokaryotic organisms have been intensively studied in terms of pathophysiological functions and molecular architecture of transmembrane pores. These proteins are also being developed for various analytical applications such as detector of proteins and DNA by engineering the structure of the pore. Staphylococcal gamma-hemolysin (Hlg), a pore-forming protein, which consists of two separate proteins, LukF and Hlg2, has potential to be a useful tool as a multifunctional biosensor. However, the fine structure of the Hlg pore has not been clarified. Our previous studies revealed that LukF and Hlg2 assemble alternately on the membrane in a molar ratio of 3:4 and 4:3 and form cylindrical heteroheptameric transmembrane pores. In the present study, we conducted quantitative analysis of the subunit arrangement of the pore by using two-dimensional (2-D) image analysis based on high-resolution transmission electron microscopy (TEM) images. Results of this study suggest a new aspect of the characteristic structure in two-component pore-forming protein and can contribute to the engineering of the Hlg pore.

Original languageEnglish
Title of host publicationBiomedical and Biotechnology Engineering; Nanoengineering for Medicine and Biology
PublisherAmerican Society of Mechanical Engineers (ASME)
Pages399-406
Number of pages8
ISBN (Print)9780791854884
DOIs
Publication statusPublished - 2011 Jan 1
EventASME 2011 International Mechanical Engineering Congress and Exposition, IMECE 2011 - Denver, CO, United States
Duration: 2011 Nov 112011 Nov 17

Publication series

NameASME 2011 International Mechanical Engineering Congress and Exposition, IMECE 2011
Volume2

Other

OtherASME 2011 International Mechanical Engineering Congress and Exposition, IMECE 2011
CountryUnited States
CityDenver, CO
Period11/11/1111/11/17

ASJC Scopus subject areas

  • Mechanical Engineering

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