Quantitative analysis of pattern of gonial proliferation during sexual maturation in Japanese scallop Patinopecten yessoensis

Makoto Osada, Satoshi Nakamura, Akihiro Kijima

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

This study was undertaken to describe and quantitatively analyze the pattern of development of gametes in the Japanese scallop Patinopecten yessoensis. Incorporation of 5-bromo-2′-deoxyuridine (BrdU) into gonial cells was used to quantitatively detect mitotically active gonial cells. Oogonia increased in number from November to December and decreased rapidly to March. A small number of oocytes was detected in November. Oocytes steadily increased in number and size up to March. The number of spermatogonia slightly increased from November to December, and increased markedly from January to March. Both ratios of BrdU-immunopositive gonial cells in the ovary and testis to gonial cells moderately increased from September to December. The ratio of BrdU-immunopositive spermatogonia to gonial cells drastically increased from January to February and kept an elevated level in March, whereas the oogonia started to disappear in January. The results suggest that the pattern of proliferation of gonial cells can be divided into two phases: (i) phase I, oogonia and spermatogonia slowly proliferate through the growing stage; and (ii) phase II, oogonia develop into oocytes and spermatogonia begin to proliferate rapidly through the mature and spawning stages. The proliferation of gonial cells is likely under different endocrine controls in phases I and II.

Original languageEnglish
Pages (from-to)1318-1324
Number of pages7
JournalFisheries Science
Volume73
Issue number6
DOIs
Publication statusPublished - 2007 Dec 1

Keywords

  • Gametogenesis
  • Gonial proliferation
  • Oogonium
  • Scallop
  • Spermatogonium

ASJC Scopus subject areas

  • Aquatic Science

Fingerprint Dive into the research topics of 'Quantitative analysis of pattern of gonial proliferation during sexual maturation in Japanese scallop Patinopecten yessoensis'. Together they form a unique fingerprint.

Cite this