TY - JOUR
T1 - Quantitative analysis of nuclear shape in oral squamous cell carcinoma is useful for predicting the chemotherapeutic response
AU - Ogura, Maki
AU - Yamamoto, Yoichiro
AU - Miyashita, Hitoshi
AU - Kumamoto, Hiroyuki
AU - Fukumoto, Manabu
N1 - Publisher Copyright:
© The Japanese Society for Clinical Molecular Morphology 2015.
PY - 2016/6/1
Y1 - 2016/6/1
N2 - The number of people afflicted with oral carcinoma in Japan has increased in recent years. Although preoperative neoadjuvant therapy with cisplatin and 5-fluorouracil are performed, chemotherapeutic response varies widely among the patients. With the aim of establishing novel indices to predict the therapeutic response to chemotherapy, we investigated the relationship between morphological features of pre-treatment oral carcinoma nuclei and the chemotherapeutic response using quantifying morphology of cell nuclei in pathological specimen images. We measured 4 morphological features of the nucleus of oral squamous cell carcinoma cases classified by the response to chemotherapy: No Change (NC) group, Partial Response (PR) group and Complete Response (CR) group. Furthermore, we performed immunohistochemical staining for p53 and Ki67 and calculated their positive rates in cancer tissues. Compactness and symmetry of the nucleus were significantly higher and nuclear edge response was significantly lower in cancer cells with lower chemotherapeutic responses compared high chemotherapeutic responders. As for positive rates of p53 and Ki67, there were no significant differences between any of the response groups. Morphological features of cancer cell nuclei in pathological specimens are sensitive predictive factors for the chemotherapeutic response to oral squamous cell carcinoma.
AB - The number of people afflicted with oral carcinoma in Japan has increased in recent years. Although preoperative neoadjuvant therapy with cisplatin and 5-fluorouracil are performed, chemotherapeutic response varies widely among the patients. With the aim of establishing novel indices to predict the therapeutic response to chemotherapy, we investigated the relationship between morphological features of pre-treatment oral carcinoma nuclei and the chemotherapeutic response using quantifying morphology of cell nuclei in pathological specimen images. We measured 4 morphological features of the nucleus of oral squamous cell carcinoma cases classified by the response to chemotherapy: No Change (NC) group, Partial Response (PR) group and Complete Response (CR) group. Furthermore, we performed immunohistochemical staining for p53 and Ki67 and calculated their positive rates in cancer tissues. Compactness and symmetry of the nucleus were significantly higher and nuclear edge response was significantly lower in cancer cells with lower chemotherapeutic responses compared high chemotherapeutic responders. As for positive rates of p53 and Ki67, there were no significant differences between any of the response groups. Morphological features of cancer cell nuclei in pathological specimens are sensitive predictive factors for the chemotherapeutic response to oral squamous cell carcinoma.
KW - Chemotherapy
KW - Digital pathology
KW - Morphology
KW - Oral squamous cell carcinoma
KW - Quantitative analysis
UR - http://www.scopus.com/inward/record.url?scp=84944622874&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84944622874&partnerID=8YFLogxK
U2 - 10.1007/s00795-015-0121-4
DO - 10.1007/s00795-015-0121-4
M3 - Article
C2 - 26439725
AN - SCOPUS:84944622874
VL - 49
SP - 76
EP - 82
JO - Medical Molecular Morphology
JF - Medical Molecular Morphology
SN - 1860-1480
IS - 2
ER -