Quantitative analysis of biofilm formation of methicillin-resistant Staphylococcus aureus (MRSA) strains from patients with orthopaedic device-related infections

Hideki Kawamura, Junichiro Nishi, Naoko Imuta, Koichi Tokuda, Hiroaki Miyanohara, Teruto Hashiguchi, Michihisa Zenmyo, Takuya Yamamoto, Kosei Ijiri, Yoshifumi Kawano, Setsuro Komiya

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Biofilms play a pivotal role in medical device-related infections. However, epidemiological analysis of biofilm formation and genotyping among clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates from patients with orthopaedic infections has rarely been reported. A total of 168 MRSA strains were examined: 23 strains from patients with device-related infection (the device group); 55 from patients with device-non-related infection (the nondevice group); and 90 from asymptomatic nasal carriers (the colonization group). Pulsed-field gel electrophoresis analysis and five genotyping methods including agr typing were performed. Biofilm formation was quantified using a microtitre plate assay. The device group had a significantly higher incidence of agr-2 than the colonization group (78.3% vs. 34.4%, P=0.001). The biofilm index of the agr-2 (0.523 ± 0.572) strains was significantly higher than those of agr-1 (0.260 ± 0.418, P<0.0001) and agr-3 (0.379 ± 0.557, P=0.045). The prevalence of strong biofilm formers in the device group (43.5%) was significantly higher than that in the nondevice group (12.7%, P=0.003) and the colonization group (20.0%, P=0.020). agr-2 MRSA strains may be more likely to cause orthopaedic device infection because of their strong biofilm formation ability.

Original languageEnglish
Pages (from-to)10-15
Number of pages6
JournalFEMS Immunology and Medical Microbiology
Volume63
Issue number1
DOIs
Publication statusPublished - 2011 Oct 1

Keywords

  • Biofilm
  • Genotyping
  • MRSA
  • Medical device
  • Orthopaedics

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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