Quantitative analysis of 3,4-dimethylmethcathinone in blood and urine by liquid chromatography-tandem mass spectrometry in a fatal case

Kiyotaka Usui, Tomomi Aramaki, Masaki Hashiyada, Yoshie Hayashizaki, Masato Funayama

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We report here the quantitative analysis of cathinone-type designer drug 3,4-dimethylmethcathinone (3,4-DMMC) in blood and urine using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in a fatal case. Abuse of 3,4-DMMC is widespread and a global issue. However, to date, there have been no reports of 3,4-DMMC-related deaths. We encountered a death in which 3,4-DMMC was thought to play a causative role, and successfully identified this designer drug from biological samples by using LC-MS/MS and QuEChERS (quick, easy, cheap, effective, rugged and safe) extraction method. For standard samples, detection of 3,4-DMMC in human blood and urine samples in the calibration range (5-400. ng/mL) was successful with recoveries of 85.9-89.4% (blood) and 95.8-101% (urine), limits of detection of 1.03 (blood) and 1.37. ng/mL (urine) and limits of quantification of 5.00 (blood) and 5.38. ng/mL (urine). The concentrations of 3,4-DMMC in blood (external iliac vein) and urine in the case were 27. mg/L and 7.6. mg/L, respectively. Some metabolites, including 3,4-dimethylcathione (DMC) and β-ketone reduced metabolites (β-OH-DMMC and β-OH-DMC), were detected in both blood and urine.

Original languageEnglish
Pages (from-to)222-226
Number of pages5
JournalLegal Medicine
Volume16
Issue number4
DOIs
Publication statusPublished - 2014 Jul

Keywords

  • 3,4-Dimethylmethcathinone
  • Designer drugs
  • Drugs of abuse
  • LC-MS/MS
  • QuEChERS
  • Synthetic cathinones

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Issues, ethics and legal aspects

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