Quantitative Analyses of Förster Resonance Energy Transfer between Identical Pyrene Chromophores (Homo-FRET) In DNA Scaffolds.

Hiromu Kashida, Hayato Kawai, Hidenori Azuma, Yasuyuki Araki, Takehiko Wada, Hiroyuki Asanuma

Research output: Contribution to journalArticlepeer-review

Abstract

Förster resonance energy transfer between identical chromophores (homo-FRET) has been difficult to analyze since neither emission intensity nor lifetime changes with the occurrence of homo-FRET. Herein we used a DNA scaffold to analyze homo-FRET between pyrene moieties. The DNA scaffold was modified with two pyrenes and a quencher, anthraquinone. Homo-FRET was detected by monitoring quenching of pyrene emission and the decrease in the fluorescence lifetime of pyrene. Homo-FRET efficiencies could be calculated by excluding effects of hetero-FRET. The experimentally determined efficiencies showed an excellent agreement with Förster theory. These results will inform design of novel molecular probes and light-harvesting antennae.

Original languageEnglish
Pages (from-to)167-172
Number of pages6
JournalChemPhotoChem
Volume5
Issue number2
DOIs
Publication statusPublished - 2021 Feb

Keywords

  • DNA
  • FRET
  • energy migration
  • energy transfer
  • pyrene

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Analytical Chemistry

Fingerprint

Dive into the research topics of 'Quantitative Analyses of Förster Resonance Energy Transfer between Identical Pyrene Chromophores (Homo-FRET) In DNA Scaffolds.'. Together they form a unique fingerprint.

Cite this