Quantitative analyses of amount and localization of radiosensitizer gold nanoparticles interacting with cancer cells to optimize radiation therapy

Keiichiro Hatoyama, Narufumi Kitamura, Mayumi Takano-Kasuya, Masayuki Tokunaga, Takahiro Oikawa, Mineto Ohta, Yoh Hamada, Hiroshi Tada, Yoshio Kobayashi, Takashi Kamei, Kohsuke Gonda

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Previous studies showed that gold nanoparticles (AuNPs) are useful radiosensitizers which optimize radiation therapy under low-dose radiation. However, the mechanisms of AuNP radiosensitization, including the amount and localization of the AuNPs interacting with cancer cells, has not yet been quantified. To answer these questions, we prepared AuNPs conjugated with anti-human epidermal growth factor receptor type 2 (HER2) antibody via polyethylene glycol (PEG) chains (AuNP-PEG-HER2ab). AuNP-PEG-HER2ab specifically bound to the HER2-expressing cancer cells and entered the cells via endocytosis. Whether endocytosis of AuNP-PEG-HER2ab occurred had no effect on radiosensitization efficacy by AuNP-PEG-HER2ab in vitro. The radiosensitization efficacy in vitro depended on dose of AuNP-PEG-HER2ab or dose of X-ray. Moreover, AuNP-PEG-HER2ab administrated into tumor-bearing mice was localized to both the periphery of the tumor tissue and near the nuclei in cancer cells in tumor deep tissue. The localization of AuNP-PEG-HER2ab in tumor tissues was important factors for in vivo powerful radiosensitization efficacy.

Original languageEnglish
Pages (from-to)1093-1100
Number of pages8
JournalBiochemical and biophysical research communications
Volume508
Issue number4
DOIs
Publication statusPublished - 2019 Jan 22

Keywords

  • Gold nanoparticle
  • HER2
  • Low-dose radiation
  • Radiation therapy
  • Radiosensitizer

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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