TY - JOUR
T1 - Quantification of the infectivity of Cryptosporidium parvum by monitoring the oocyst discharge from SCID mice
AU - Hikosaka, Kenji
AU - Satoh, Masaaki
AU - Koyama, Yukari
AU - Nakai, Yutaka
PY - 2005/11/25
Y1 - 2005/11/25
N2 - Doses of 1-105 oocysts of Cryptosporidium parvum HNJ-1 were inoculated into severe combined immunodeficient (SCID) mice, and the discharge of oocysts was monitored for 30 days post inoculation. None of the mice discharged any oocysts after oral inoculation of one oocyst. Only one of five SCID mice discharged oocysts after oral inoculation of 10 oocysts, and the prepatent period was 17 days. The other four mice did not discharge any oocysts. All the SCID mice discharged oocysts after oral inoculation of 10 2-105 oocysts. The prepatent periods were 13-17, 8-10, 8, and 10 days in SCID mice inoculated with 102, 103, 10 4, and 105 oocysts, respectively. A proportional correlation was observed between inoculation doses of oocysts ranging from 10 to 104 oocysts and the corresponding prepatent periods. The prepatent period can be used to evaluate the infectivity of C. parvum oocysts.
AB - Doses of 1-105 oocysts of Cryptosporidium parvum HNJ-1 were inoculated into severe combined immunodeficient (SCID) mice, and the discharge of oocysts was monitored for 30 days post inoculation. None of the mice discharged any oocysts after oral inoculation of one oocyst. Only one of five SCID mice discharged oocysts after oral inoculation of 10 oocysts, and the prepatent period was 17 days. The other four mice did not discharge any oocysts. All the SCID mice discharged oocysts after oral inoculation of 10 2-105 oocysts. The prepatent periods were 13-17, 8-10, 8, and 10 days in SCID mice inoculated with 102, 103, 10 4, and 105 oocysts, respectively. A proportional correlation was observed between inoculation doses of oocysts ranging from 10 to 104 oocysts and the corresponding prepatent periods. The prepatent period can be used to evaluate the infectivity of C. parvum oocysts.
KW - Cryptosporidium parvum
KW - Experimental infection
KW - Infectivity
KW - SCID mouse
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U2 - 10.1016/j.vetpar.2005.06.017
DO - 10.1016/j.vetpar.2005.06.017
M3 - Article
C2 - 16076530
AN - SCOPUS:27544452389
VL - 134
SP - 173
EP - 176
JO - Veterinary Parasitology
JF - Veterinary Parasitology
SN - 0304-4017
IS - 1-2
ER -