Quantification of the infectivity of Cryptosporidium parvum by monitoring the oocyst discharge from SCID mice

Kenji Hikosaka, Masaaki Satoh, Yukari Koyama, Yutaka Nakai

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Doses of 1-105 oocysts of Cryptosporidium parvum HNJ-1 were inoculated into severe combined immunodeficient (SCID) mice, and the discharge of oocysts was monitored for 30 days post inoculation. None of the mice discharged any oocysts after oral inoculation of one oocyst. Only one of five SCID mice discharged oocysts after oral inoculation of 10 oocysts, and the prepatent period was 17 days. The other four mice did not discharge any oocysts. All the SCID mice discharged oocysts after oral inoculation of 10 2-105 oocysts. The prepatent periods were 13-17, 8-10, 8, and 10 days in SCID mice inoculated with 102, 103, 10 4, and 105 oocysts, respectively. A proportional correlation was observed between inoculation doses of oocysts ranging from 10 to 104 oocysts and the corresponding prepatent periods. The prepatent period can be used to evaluate the infectivity of C. parvum oocysts.

Original languageEnglish
Pages (from-to)173-176
Number of pages4
JournalVeterinary Parasitology
Volume134
Issue number1-2
DOIs
Publication statusPublished - 2005 Nov 25

Keywords

  • Cryptosporidium parvum
  • Experimental infection
  • Infectivity
  • SCID mouse

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)

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