Quantification of methanogen cell density in anaerobic granular sludge consortia by fluorescence in-situ hybridization

T. Tagawa, K. Syutsubo, Y. Sekiguchil, A. Ohashi, H. Harada

Research output: Contribution to journalConference article

27 Citations (Scopus)

Abstract

Whole cell fluorescence in-situ hybridization (FISH) with 16S rRNA targeted oligonucleotides was applied to reveal the microbial ecological structure of UASB-grown granular sludge. The FISH analysis indicated that the members of the domain Archaea accounted for 28 to 53% of the total cells in various granular sludge sources, while Methanosaeta and Methanobacteriaceae cells accounted for 13 to 38%, and 4 to 27%, respectively. Methanosaeta cell density and Methanobacteriaceae cell density were strongly correlated, respectively, with acetate-utilizing methane production activity and with hydrogen-utilizing methane production activity.

Original languageEnglish
Pages (from-to)77-82
Number of pages6
JournalWater Science and Technology
Volume42
Issue number3-4
DOIs
Publication statusPublished - 2000
EventWater Quality Management in Asia (Asian Waterqual'99) - Taipei, Taiwan
Duration: 1999 Oct 181999 Oct 20

Keywords

  • 16S rRNA
  • Anaerobic granular sludge
  • Archaea
  • Fluorescence in-situ hybridization (FISH)
  • Methanogen
  • Microbial community structure

ASJC Scopus subject areas

  • Environmental Engineering
  • Water Science and Technology

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