Quantification of intact carboplatin in human plasma ultrafitrates using hydrophilic interaction liquid chromatography-tandem mass spectrometry and its application to a pharmacokinetic study

Hajime Ito, Hiroaki Yamaguchi, Asuka Fujikawa, Narumi Shiida, Nobuaki Tanaka, Jiro Ogura, Masaki Kobayashi, Takehiro Yamada, Nariyasu Mano, Ken Iseki

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Carboplatin is a platinum agent that is used for treatment of non-small-cell lung cancer and ovarian cancer. A sensitive and selective analytical method for the quantification of carboplatin in human plasma ultrafiltrates using liquid chromatography-tandem mass spectrometry was developed. Human plasma ultrafiltrates were precipitated by acetonitrile containing carboplatin-d4 as an internal standard and were further diluted with acetonitrile. Chromatographic separation was performed on a Accucore HILIC (50. mm × 2.1. mm i.d., 2.6. μm) column using mobile phase (acetonitrile-water-acetic acid = 90:10:0.1, v/v/v) at the flow rate of 0.2. mL/min. Detection was performed on electrospray ionization triple quadrupole tandem mass spectrometer using low-energy collision induced dissociation (CID-MS/MS) analysis operating in the selected reaction monitoring (SRM) scan mode. The lower limit of quantification for carboplatin was 0.025. μg/mL. This method covered a linearity range of 0.025-50. μg/mL. The intra-day precision and inter-day precision (R.S.D.) ranged from 1.5 to 4.3%, and the accuracy (R.E.) was within ±2.9%. The present method was applied to a clinical pharmacokinetic study of carboplatin in a cancer patient.

Original languageEnglish
Pages (from-to)18-23
Number of pages6
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume917-918
DOIs
Publication statusPublished - 2013 Feb 5

Keywords

  • Carboplatin
  • Hydrophilic interaction liquid chromatography-tandem mass spectrometry
  • Plasma ultrafiltrates

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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