Quantification of cyclic electron flow in spinach leaf discs

Jiancun Kou, Shunichi Takahashi, Riichi Oguchi, Murray R. Badger, Wah Soon Chow

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

We quantified the photosynthetic cyclic electron flux (CEF) around Photosystem I as the difference between the total electron flux through PS I (ETR1) and the linear electron flux through both photosystems. Both measurements were made in the whole tissue of spinach leaf discs illuminated in the same geometry and in CO2-enriched air to suppress photorespiration. (1) CEF was negligibly small below 300 umol photons m−2s−1. Above this irradiance, CEF increased approximately linearly up to the highest irradiance used (1,900 umol photons m−2s−1). (2) CEF at a fixed irradiance of 980 μmol m−2s−1 increased by a factor of almost 3 as the temperature was increased from 5 °C to 40 °C. It did not decline, even when the linear electron flux decreased at high temperatures. (3) Antimycin A, at a high concentration, decreased CEF to about 10% of the control value without affecting the linear electron flux. This method appears to be reliable for quantifying CEF non-intrusively. By contrast, estimation of the linear electron flux from chlorophyll fluorescence over-estimated CEF in the above treatments.

Original languageEnglish
Title of host publicationAdvanced Topics in Science and Technology in China
PublisherSpringer
Pages271-274
Number of pages4
DOIs
Publication statusPublished - 2013 Jan 1
Externally publishedYes

Publication series

NameAdvanced Topics in Science and Technology in China
ISSN (Print)1995-6819
ISSN (Electronic)1995-6827

Keywords

  • Antimycin A
  • Cyclic electron flow
  • Linear electron flow
  • P700
  • Photosystem I

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Engineering(all)
  • General

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  • Cite this

    Kou, J., Takahashi, S., Oguchi, R., Badger, M. R., & Chow, W. S. (2013). Quantification of cyclic electron flow in spinach leaf discs. In Advanced Topics in Science and Technology in China (pp. 271-274). (Advanced Topics in Science and Technology in China). Springer. https://doi.org/10.1007/978-3-642-32034-7_56