Quantification of cyclic electron flow in spinach leaf discs

Jiancun Kou, Shunichi Takahashi, Riichi Oguchi, Murray R. Badger, Wah Soon Chow

    Research output: Chapter in Book/Report/Conference proceedingChapter

    3 Citations (Scopus)


    We quantified the photosynthetic cyclic electron flux (CEF) around Photosystem I as the difference between the total electron flux through PS I (ETR1) and the linear electron flux through both photosystems. Both measurements were made in the whole tissue of spinach leaf discs illuminated in the same geometry and in CO2-enriched air to suppress photorespiration. (1) CEF was negligibly small below 300 umol photons m−2s−1. Above this irradiance, CEF increased approximately linearly up to the highest irradiance used (1,900 umol photons m−2s−1). (2) CEF at a fixed irradiance of 980 μmol m−2s−1 increased by a factor of almost 3 as the temperature was increased from 5 °C to 40 °C. It did not decline, even when the linear electron flux decreased at high temperatures. (3) Antimycin A, at a high concentration, decreased CEF to about 10% of the control value without affecting the linear electron flux. This method appears to be reliable for quantifying CEF non-intrusively. By contrast, estimation of the linear electron flux from chlorophyll fluorescence over-estimated CEF in the above treatments.

    Original languageEnglish
    Title of host publicationAdvanced Topics in Science and Technology in China
    PublisherSpringer Science and Business Media Deutschland GmbH
    Number of pages4
    Publication statusPublished - 2013

    Publication series

    NameAdvanced Topics in Science and Technology in China
    ISSN (Print)1995-6819
    ISSN (Electronic)1995-6827


    • Antimycin A
    • Cyclic electron flow
    • Linear electron flow
    • P700
    • Photosystem I

    ASJC Scopus subject areas

    • Chemical Engineering(all)
    • Engineering(all)
    • General


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