Quantification of Ca2+-activated K+ channels under hormonal control in pig pancreas acinar cells

Yoshio Maruyama, O. H. Petersen, P. Flanagan, G. T. Pearson

Research output: Contribution to journalArticlepeer-review

140 Citations (Scopus)

Abstract

Ca2+- and voltage-activated K+ channels are found in many electrically excitable cells and have an important role in regulating electrical activity1-4. Recently, the large K+ channel has been found in the baso-lateral plasma membranes of salivary gland acinar cells, where it may be important in the regulation of salt transport5. Using patch-clamp methods6,7 to record single-channel currents from excised fragments of baso-lateral acinar cell membranes in combination with current recordings from isolated single acinar cells and two- and three-cell clusters, we have now for the first time characterized the K+ channels quantitatively. In pig pancreatic acini there are 25-60 K+ channels per cell with a maximal single channel conductance of about 200 pS. We have quantified the relationship between internal ionized Ca2+ concentration ([Ca2+]i) membrane potential and open-state probability (p) of the K+ channel. By comparing curves obtained from excised patches relating membrane potential to p, at different levels of [Ca2+]i, with similar curves obtained from intact cells, [Ca2+]i in resting acinar cells was found to be between 10-8 and 10-7 M. In microelectrode experiments acetylcholine (ACh), gastrin-cholecystokinin (CCK) as well as bombesin peptides evoked Ca2+-dependent opening of the K+ conductance pathway, resulting in membrane hyperpolarization. The large K+ channel, which is under strict dual control by internal Ca2+ and voltage, may provide a crucial link between hormone-evoked increase in internal Ca2+ concentration and the resulting NaCl-rich fluid secretion 5,8.

Original languageEnglish
Pages (from-to)228-232
Number of pages5
JournalNature
Volume305
Issue number5931
DOIs
Publication statusPublished - 1983 Dec 1

ASJC Scopus subject areas

  • General

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