Quality evaluation of in vitro-produced bovine embryos by respiration measurment and development of semi-automatic instrument

Shigeo Aoyagi, Yousuke Utsumi, Masaaki Matsudaira, Hiroshi Yamada, Masayuki Kacchi, Hitoshi Shiku, A. B.E. Hiroyuki, Hiroyoshi Hoshi, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The quality of in vitro-produced bovine embryos was investigated by a scanning electrochemical microscopy (SECM)-based instrument for measuring the respiratory activity of individual bovine embryos. The measurement, was performed by using a measuring cell equipped with a six cone-shaped microwell, a temperature-controlling unit, an auto-approach program to noninvasively locate the probe-microelectrode very close to the sample bovine embryo, a program for semi-automatic probe-scanning, and analyzing software to estimate the respiration activity of a single bovine embryo based on a spherical diffusion theory.

Original languageEnglish
Pages (from-to)847-854
Number of pages8
JournalBUNSEKI KAGAKU
Volume55
Issue number11
DOIs
Publication statusPublished - 2006

Keywords

  • Bovine embryo
  • Measuring respiration
  • Quality evaluation
  • Respiration rate

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Quality evaluation of in vitro-produced bovine embryos by respiration measurment and development of semi-automatic instrument'. Together they form a unique fingerprint.

Cite this