Abstract
One hundred sixty-one EST-SNP markers were newly developed for analysis of QTLs for resistance to black rot caused by Xanthomonas campestris pv. campestris by determining EST sequences of a resistant line obtained from cabbage 'Early Fuji' and a susceptible broccoli line. A linkage map consisting of nine linkage groups was constructed with a total of 209 markers, including these new SNP markers and previously reported DNA markers. F2 plants grown in a field for 1 month were inoculated by spraying bacteria of race 1, and disease severity of each plant was recorded. Three QTLs, i. e., QTL-1, QTL-2, and QTL-3, were detected on linkage group C2, C4 and C5, respectively. QTL-1, which showed the highest LOD score and additive effect, was again detected in another F2 population used the next year, suggesting QTL-1 to be a major QTL. QTL-2 and QTL-3 could be minor QTLs influenced by environmental factors. The genomic region harboring QTL-1 showed synteny with a region from 5.3 to 7.4 Mb from the short arm end of chromosome 5 of Arabidopsis thaliana, which is rich in TIR-NBS-LRR family genes. The identified SNP markers in QTL-1 are considered to be useful in marker-assisted selection for black rot resistance in Brassica oleracea lines.
Original language | English |
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Pages (from-to) | 289-295 |
Number of pages | 7 |
Journal | Euphytica |
Volume | 190 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2013 Jan 1 |
Keywords
- Brassica oleracea
- Dot-blot-SNP markers
- Marker-assisted selection
- Synteny
- Xanthomonas campestris pv. campestris
ASJC Scopus subject areas
- Agronomy and Crop Science
- Genetics
- Plant Science
- Horticulture