QTL analysis of black rot resistance in cabbage using newly developed EST-SNP markers

Yasuko Kifuji, Hideaki Hanzawa, Yuuichi Terasawa, Ashutosh, Takeshi Nishio

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

One hundred sixty-one EST-SNP markers were newly developed for analysis of QTLs for resistance to black rot caused by Xanthomonas campestris pv. campestris by determining EST sequences of a resistant line obtained from cabbage 'Early Fuji' and a susceptible broccoli line. A linkage map consisting of nine linkage groups was constructed with a total of 209 markers, including these new SNP markers and previously reported DNA markers. F2 plants grown in a field for 1 month were inoculated by spraying bacteria of race 1, and disease severity of each plant was recorded. Three QTLs, i. e., QTL-1, QTL-2, and QTL-3, were detected on linkage group C2, C4 and C5, respectively. QTL-1, which showed the highest LOD score and additive effect, was again detected in another F2 population used the next year, suggesting QTL-1 to be a major QTL. QTL-2 and QTL-3 could be minor QTLs influenced by environmental factors. The genomic region harboring QTL-1 showed synteny with a region from 5.3 to 7.4 Mb from the short arm end of chromosome 5 of Arabidopsis thaliana, which is rich in TIR-NBS-LRR family genes. The identified SNP markers in QTL-1 are considered to be useful in marker-assisted selection for black rot resistance in Brassica oleracea lines.

Original languageEnglish
Pages (from-to)289-295
Number of pages7
JournalEuphytica
Volume190
Issue number2
DOIs
Publication statusPublished - 2013 Jan 1

Keywords

  • Brassica oleracea
  • Dot-blot-SNP markers
  • Marker-assisted selection
  • Synteny
  • Xanthomonas campestris pv. campestris

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science
  • Horticulture

Fingerprint Dive into the research topics of 'QTL analysis of black rot resistance in cabbage using newly developed EST-SNP markers'. Together they form a unique fingerprint.

Cite this