Pyruvate as a fluorescence quencher: A new spectroscopic assay for pyruvate reactions

Toshio Ando, Hidetake Miyata

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Pyruvate ion, which is biologically ubiquitous and participates in many metabolic reactions, was found to be an effective quencher of fluorescence. Compared to other negatively charged quenchers such as I-, pyruvate is not toxic to proteins. By adding an inert, long-lived fluorophore to systems transacting pyruvate, it is possible to estimate activity by measuring the time course of the change in pyruvate quenching of the fluorophore. The procedure is illustrated by measuring the myosin subfragment-1 ATPase activity with a high sensitivity.

Original languageEnglish
Pages (from-to)170-175
Number of pages6
JournalAnalytical Biochemistry
Volume129
Issue number1
DOIs
Publication statusPublished - 1983 Feb 15
Externally publishedYes

Keywords

  • ATPase assay
  • charged quencher
  • fluorescence quenching
  • pyruvate
  • pyruvate reactions

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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