Puromycin insensitive leucyl-specific aminopeptidase (PILSAP) affects RhoA activation in endothelial cells

Takahiro Suzuki, Mayumi Abe, Hiroki Miyashita, Toshimitsu Kobayashi, Yasufumi Sato

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Puromycin insensitive leucyl-specific aminopeptidase (PILSAP) expressed in endothelial cells (ECs) plays an important role in angiogenesis due to its involvement in migration, proliferation and network formation. Here we examined the biological function of PILSAP with respect to EC morphogenesis and the related intracellular signaling for this process. When mouse endothelial MSS31 cells were cultured, a dominant negative PILSAP mutant converted cell shape to disk-like morphology, blocked stress fiber formation, and augmented membrane ruffling in random directions. These phenotypic changes led us to test whether PILSAP affected activities of Rho family small G-proteins. Abrogation of PILSAP enzymatic activity or its expression attenuated RhoA but not Rac1 activation during cell adhesion. This attenuation of RhoA activation was also evident when G-protein coupled receptors such as proteinase-activated receptor or lysophosphatidic acid receptor were activated in ECs. These results indicate that PILSAP affects RhoA activation and that influences the proper function of ECs.

Original languageEnglish
Pages (from-to)708-715
Number of pages8
JournalJournal of Cellular Physiology
Volume211
Issue number3
DOIs
Publication statusPublished - 2007 Jun

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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