Purification of the 200 kDa hemocyte membrane protein of Sarcophaga peregrina and its specific interaction with fat body

Hideaki Kobayashi, Shoichiro Kurata, Shunji Natori

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

A 200 kDa protein specifically expressed on the surface of pupal hemocytes of Sarcophaga peregrina was purified from the hemocyte membrane. This protein has been suggested to participate in dissociation of the fat body in the pupal stage of this insect. This protein was found to inhibit the dissociation of the fat body in vitro. Furthermore, it was shown to bind to the fat body and the binding could be saturated. These results suggested that pupal hemocytes expressing the 200 kDa protein interact directly with specific binding sites on the basement membrane of the fat body when they disintegrate this tissue.

Original languageEnglish
Pages (from-to)517-522
Number of pages6
JournalInsect Biochemistry
Volume21
Issue number5
DOIs
Publication statusPublished - 1991
Externally publishedYes

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