Purification of flounder (Paralichthys olivaceus) fibronectin and its localization during re‐epithelialization at a fin wound

T. Kurokawa, T. Suzuki, S. Funakoshi

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Plasma fibronectin (FN) of flounder Paralichthys olivacem was purified and characterized. Flounder FN was purified by a combination of affinity chromatography using Sepharose coupled with flounder gelatin and gel filtration on Superose 6. Flounder FN was found to be a disulphide‐linked heterodimer of 220 and 230 kDa polypeptides. It had cell‐spreading activity for baby hamster kidney (BHK) cells, which was inhibited by the synthetic peptide, Gly‐Arg‐Gly‐Asp‐Ser‐Pro. In flounder explants, anti‐flounder FN antiserum distinguished fibroblast‐like cells from epithelial cells; indirect immunofluorescence showed that the fibroblast‐like cells exhibited a fibrous staining to the antiserum, but that epithelial cells did not. These results suggest that flounder FN is a homologue of mammalian FN. The localization of FN during re‐epithelialization at the site of a severed fin was investigated. When the top of the fin was cut, epidermal cells covered the surface of the wound within 1 h. FN is detected at the wound site during epidermal cell migration, suggesting that it serves as a cell‐adhesion factor for prompt re‐epithelialization at wound sites.

Original languageEnglish
Pages (from-to)421-432
Number of pages12
JournalJournal of Fish Biology
Volume43
Issue number3
DOIs
Publication statusPublished - 1993 Sep

Keywords

  • Paralichthys olivaceus
  • fin fibronectin
  • immunohistochemistry
  • re‐epithelialization
  • wound healing

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

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