Purification of ca2+/calmodulin-dependent protein kinase ii from rat stomach and its localization in the mucosa

Wei Jun Wang, Ko HJ1 Fukunaga, Satoshi Goto, Eishichi MIYAMoTo

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Abstract

Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) with a Mr of 530,000 was purified from rat stomach. The enzyme showed a major protein band with 52 kDa and minor components with 50 and 54 kDa when examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The physicochemical and kinetic properties of the enzyme were similar to those of brain CaM kinase II. The enzyme showed a broad substrate specificity, and wascapable of phosphorylating several endogenous proteins in the cytosol and membrane fractions.The polyclonal antibody against brain CaM kinase II cross-reacted with the enzyme.Strong immunoreactivity was observed in the parietal cells of stomach by the immunohistochemical investigation. These results suggest that CaM kinase II regulates the Ca2+-dependent cellular functions of the parietal cells.

Original languageEnglish
Pages (from-to)231-240
Number of pages10
JournalBiomedical Research (Japan)
Volume15
Issue number4
DOIs
Publication statusPublished - 1994 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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