Purification of a 200 kDa protein-binding protein from the fat body of Sarcophaga peregrina larvae

Hideaki Kobayashi, Shoichiro Kurata, Shunji Natori

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Previously, we demonstrated that pupal hemocytes of Sarcophaga disintegrate the larval fat body in vitro, mimicking the fat body decomposition that takes place in the pupal stage (Kurata S., Komano H. and Natori S. (1989) J. Insect Physiol. 35, 559-565), and that hemocytes expressing a 200 kDa protein appear only in the pupal stage (Kurata S., Kobayashi H. and Natori S. (1991) Dev. Biol. 146, 179-185). In the present study, we purified a protein from the larval fat body that specifically binds to the hemocyte 200 kDa protein. This binding protein consisted of 220 and 230 kDa subunits. This binding protein disappeared from the fat body within 10 h after pupation, suggesting that the hemocytes expressing the 200 kDa protein interact with the fat body via this binding protein during this period.

Original languageEnglish
Pages (from-to)393-399
Number of pages7
JournalInsect Biochemistry and Molecular Biology
Volume25
Issue number3
DOIs
Publication statusPublished - 1995 Mar
Externally publishedYes

Keywords

  • 200 kDa Protein
  • Binding protein
  • Decomposition
  • Fat body
  • Metamorphosis
  • Sarcophaga

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Insect Science

Fingerprint Dive into the research topics of 'Purification of a 200 kDa protein-binding protein from the fat body of Sarcophaga peregrina larvae'. Together they form a unique fingerprint.

  • Cite this