Purification and storage of ribulose 1,5-bisphosphate carboxylase from rice leaves

Amane Makino, Tadahiko Mae, Koji Ohira

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

Ribulose 1,5-bisphosphate (RuBP) carboxylase was purified from rice leaves. By using a buffer containing 12.5% (v/v) glycerol throughout purification, the enzyme was protected from cold lability and was obtained at a high yield (5.5 mg/g fresh wt). The purified enzyme exhibited different rates of CO2/Mg2+-activation by temperature pretreatment/storage.The purified enzyme was stable for at least one year in phosphate buffer containing 12.5% (v/v) glycerol at 4°C or 50% (v/v) glycerol at -20°C.

Original languageEnglish
Pages (from-to)1169-1173
Number of pages5
JournalPlant and Cell Physiology
Volume24
Issue number6
Publication statusPublished - 1983 Sep 1

Keywords

  • Oryza saliva
  • Ribulose 1,5-bisphosphate carboxylase

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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