1. 1. Two anionic enzymes, designated as trypsins 1 and 2, were purified from the pancreas of the eel Anguilla japonica by DEAE-cellulose column chromatography and Sephadex G-75 gel filtration. 2. 2. The final preparation of trypsin 1 was homogeneous but that of trypsin 2 still contained impurities. 3. 3. Both enzymes had similar pH optima of near 8.3 for the hydrolysis of N-tosyl-l-arginine methyl ester. 4. 4. Trypsin 1 was stabilized by calcium ions but the stability of trypsin 2 was not affected by calcium ions. 5. 5. Both enzymes were inhibited by typical trypsin inhibitors including serine proteinase inhibitors.
|Number of pages||5|
|Journal||Comparative Biochemistry and Physiology -- Part B: Biochemistry and|
|Publication status||Published - 1985|
ASJC Scopus subject areas
- Molecular Biology