TY - JOUR
T1 - Purification and properties of agglutinins from conger eel, Conger myriaster (Brevoort), skin mucus
AU - Kamiya, H.
AU - Muramoto, K.
AU - Goto, R.
N1 - Funding Information:
supported by a grant from Nissan Science Foundation.
PY - 1988
Y1 - 1988
N2 - The skin mucus of the conger eel, Conger myriaster, contains galactose-specific agglutinins. Hemagglutinating activity is independent of divalent cations and is destroyed by heating at 50 °C for 15 min. The mucus agglutinins, named congerins, are a mixture of proteins with different electrical charges. Three of these molecules were isolated by affinity chromatography on acid-treated Sepharose 4B and by ion-exchange chromatography. They are simple proteins with the same molecular weight of 30,000 and consisting of two subunits (each 13,000 daltons). The agglutinins inhibited the normal embryonic development of the starfish Asterina pectinifera, and lysed the fertilized eggs at a concentration of 25 μg protein/ml. They also agglutinated but did not inhibit the growth of a marine bacterium, Vibrio anguillarum.
AB - The skin mucus of the conger eel, Conger myriaster, contains galactose-specific agglutinins. Hemagglutinating activity is independent of divalent cations and is destroyed by heating at 50 °C for 15 min. The mucus agglutinins, named congerins, are a mixture of proteins with different electrical charges. Three of these molecules were isolated by affinity chromatography on acid-treated Sepharose 4B and by ion-exchange chromatography. They are simple proteins with the same molecular weight of 30,000 and consisting of two subunits (each 13,000 daltons). The agglutinins inhibited the normal embryonic development of the starfish Asterina pectinifera, and lysed the fertilized eggs at a concentration of 25 μg protein/ml. They also agglutinated but did not inhibit the growth of a marine bacterium, Vibrio anguillarum.
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U2 - 10.1016/0145-305X(88)90007-9
DO - 10.1016/0145-305X(88)90007-9
M3 - Article
C2 - 3384160
AN - SCOPUS:0023969635
VL - 12
SP - 309
EP - 318
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
SN - 0145-305X
IS - 2
ER -