Purification and properties of agglutinins from conger eel, Conger myriaster (Brevoort), skin mucus

H. Kamiya, K. Muramoto, R. Goto

    Research output: Contribution to journalArticle

    72 Citations (Scopus)

    Abstract

    The skin mucus of the conger eel, Conger myriaster, contains galactose-specific agglutinins. Hemagglutinating activity is independent of divalent cations and is destroyed by heating at 50 °C for 15 min. The mucus agglutinins, named congerins, are a mixture of proteins with different electrical charges. Three of these molecules were isolated by affinity chromatography on acid-treated Sepharose 4B and by ion-exchange chromatography. They are simple proteins with the same molecular weight of 30,000 and consisting of two subunits (each 13,000 daltons). The agglutinins inhibited the normal embryonic development of the starfish Asterina pectinifera, and lysed the fertilized eggs at a concentration of 25 μg protein/ml. They also agglutinated but did not inhibit the growth of a marine bacterium, Vibrio anguillarum.

    Original languageEnglish
    Pages (from-to)309-318
    Number of pages10
    JournalDevelopmental and Comparative Immunology
    Volume12
    Issue number2
    DOIs
    Publication statusPublished - 1988 Jan 1

    ASJC Scopus subject areas

    • Immunology
    • Developmental Biology

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