TY - JOUR
T1 - Purification and properties of β-1,4-xylanase from Aeromonas caviae W-61
AU - Viet, D. N.
AU - Kamio, Y.
AU - Abe, N.
AU - Kaneko, J.
AU - Izaki, K.
PY - 1991
Y1 - 1991
N2 - Aeromonas caviae W-61, which was isolated from water samples at the Faculty of Agriculture, Tohoku University, produced β-1,4-xylanase (1,4-β-D-xylan xylanohydrolase; EC 3.2.1.8) extracellularly. The xylanase was purified to homogeneity by using DEAE-Sephadex A-50, CM-Sephadex C-50, and Sephadex G-100 column chromatographies. The molecular weight of the purified enzyme was estimated to be 22,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The isoelectric point of the enzyme was 9.2. The optimal pH and temperature for the activity of the enzyme were 7.0 and 55°C, respectively. The enzyme was stable at pH 7.0 at temperatures of up to 50°C. As enzymatic products, various xylo-oligosaccharides such as xylobiose, xylotriose, xylotetraose, and xylopentaose were formed, and only a small amount of xylose was detected. The purified enzyme did not hydrolyze starch, cellulose, carboxymethylcellulose, or β-1,3-xylan.
AB - Aeromonas caviae W-61, which was isolated from water samples at the Faculty of Agriculture, Tohoku University, produced β-1,4-xylanase (1,4-β-D-xylan xylanohydrolase; EC 3.2.1.8) extracellularly. The xylanase was purified to homogeneity by using DEAE-Sephadex A-50, CM-Sephadex C-50, and Sephadex G-100 column chromatographies. The molecular weight of the purified enzyme was estimated to be 22,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The isoelectric point of the enzyme was 9.2. The optimal pH and temperature for the activity of the enzyme were 7.0 and 55°C, respectively. The enzyme was stable at pH 7.0 at temperatures of up to 50°C. As enzymatic products, various xylo-oligosaccharides such as xylobiose, xylotriose, xylotetraose, and xylopentaose were formed, and only a small amount of xylose was detected. The purified enzyme did not hydrolyze starch, cellulose, carboxymethylcellulose, or β-1,3-xylan.
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U2 - 10.1128/aem.57.2.445-449.1991
DO - 10.1128/aem.57.2.445-449.1991
M3 - Article
C2 - 16348410
AN - SCOPUS:0026011633
VL - 57
SP - 445
EP - 449
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 2
ER -