Purification and molecular cloning of cDNA for an inducible antibacterial protein from larvae of the coleopteran, Tenebrio molitor

Hyun Joo Moon, So Young Lee, Shoichiro Kurata, Shunji Natori, Bok Luel Lee

Research output: Contribution to journalArticlepeer-review

70 Citations (Scopus)

Abstract

Antibacterial activity was induced in the hemolymph of larvae of the coleopteran Tenebrio molitor by injection of Escherichia coli* An antibacterial protein, named tenecin 1, was purified to homogeneity from the larval hemolymph and characterized. A cDNA clone for tenecin 1 was isolated and its complete sequence was determined. This protein was found to inhibit the growth of Gram-positive bacteria and to consist of 43-amino acid residues including six cysteine residues. The disulfide structure of tenecin 1 was determined by sequencing cysteine containing peptides obtained by digesting tenecin 1 with endopep-tidase Lys-C, trypsin, and thermolysin. The amino acid sequence and its disulfide bonds were similar to those of sapecin and sapecin C, antibacterial proteins of Sarcophaga peregrina.

Original languageEnglish
Pages (from-to)53-58
Number of pages6
JournalJournal of biochemistry
Volume116
Issue number1
DOIs
Publication statusPublished - 1994 Jul
Externally publishedYes

Keywords

  • Antibacterial protein
  • Coleopteran insect
  • Inducible protein
  • Molecular cloning
  • Purification

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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