We have demonstrated that small GTPase Rab27 regulates dense-granule secretion in platelets. Using Rab27a affinity chromatography, we purified Munc13-4 as a novel Rab27a interacting protein from platelet cytosol. This chapter describes the purification of Munc13-4 and an in vitro assay system analyzing the mechanism of dense-granule secretion in platelets. The activity of Munc13-4 is tested in this assay.
ASJC Scopus subject areas
- Molecular Biology