Purification and functional analysis of a Rab27 effector Munc13-4 Using a semiintact platelet dense-granule secretion assay

Ryutaro Shirakawa, Tomohito Higashi, Hirokazu Kondo, Akira Yoshioka, Toru Kita, Hisanori Horiuchi

Research output: Contribution to journalReview articlepeer-review

10 Citations (Scopus)

Abstract

We have demonstrated that small GTPase Rab27 regulates dense-granule secretion in platelets. Using Rab27a affinity chromatography, we purified Munc13-4 as a novel Rab27a interacting protein from platelet cytosol. This chapter describes the purification of Munc13-4 and an in vitro assay system analyzing the mechanism of dense-granule secretion in platelets. The activity of Munc13-4 is tested in this assay.

Original languageEnglish
Article number67
Pages (from-to)778-788
Number of pages11
JournalMethods in enzymology
Volume403
DOIs
Publication statusPublished - 2005 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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