Purification and Characterization of the Lectins of the Soft Coral Lobophytum variatum

Rina Goto-Nance; Koji Muramoto; Yohko Zenpo; Hisao Kamiya

doi:10.2331/fishsci.62.297

Purification and Characterization of the Lectins of the Soft Coral Lobophytum variatum

Rina Goto-Nance, Koji Muramoto, Yohko Zenpo, Hisao Kamiya

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The extract of the soft coral Lobophytum variatum agglutinated horse erythrocytes but not human ABO or rabbit erythrocytes in the presense of calcium ions. The activity was inhibited by mucin Type I from the bovine submaxillary gland and also by simple sugars such as D-ribose and N-acetylneuraminic acid. Two lectins, LVL-1 and LVL-2, were purified by gel filtration on Sepharose 4B succeeded by ion-exchange chromatography on Mono-Q. Both lectins were glycoproteins composed of covalently bonded subunits of 53 kDa. The sequence of the amino-terminal region of LVL-1 was determined as Ala-Ile-Asn-Gln-Ser-Ser-Gly-Asn-Leu-(X)-Asp-Arg-Leu-Gln-Glu-Arg-Phe-(X)-Leu-Asp- His-, where X is an unidentified residue.

Original language	English
Pages (from-to)	297-301
Number of pages	5
Journal	Fisheries Science
Volume	62
Issue number	2
DOIs	https://doi.org/10.2331/fishsci.62.297
Publication status	Published - 1996 Apr

Keywords

Agglutinin
Hemagglutinin
Lectin
Lobophytum variatum
Soft coral

ASJC Scopus subject areas

Aquatic Science

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10.2331/fishsci.62.297

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title = "Purification and Characterization of the Lectins of the Soft Coral Lobophytum variatum",

abstract = "The extract of the soft coral Lobophytum variatum agglutinated horse erythrocytes but not human ABO or rabbit erythrocytes in the presense of calcium ions. The activity was inhibited by mucin Type I from the bovine submaxillary gland and also by simple sugars such as D-ribose and N-acetylneuraminic acid. Two lectins, LVL-1 and LVL-2, were purified by gel filtration on Sepharose 4B succeeded by ion-exchange chromatography on Mono-Q. Both lectins were glycoproteins composed of covalently bonded subunits of 53 kDa. The sequence of the amino-terminal region of LVL-1 was determined as Ala-Ile-Asn-Gln-Ser-Ser-Gly-Asn-Leu-(X)-Asp-Arg-Leu-Gln-Glu-Arg-Phe-(X)-Leu-Asp- His-, where X is an unidentified residue.",

keywords = "Agglutinin, Hemagglutinin, Lectin, Lobophytum variatum, Soft coral",

author = "Rina Goto-Nance and Koji Muramoto and Yohko Zenpo and Hisao Kamiya",

year = "1996",

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T1 - Purification and Characterization of the Lectins of the Soft Coral Lobophytum variatum

AU - Goto-Nance, Rina

AU - Muramoto, Koji

AU - Zenpo, Yohko

AU - Kamiya, Hisao

PY - 1996/4

Y1 - 1996/4

N2 - The extract of the soft coral Lobophytum variatum agglutinated horse erythrocytes but not human ABO or rabbit erythrocytes in the presense of calcium ions. The activity was inhibited by mucin Type I from the bovine submaxillary gland and also by simple sugars such as D-ribose and N-acetylneuraminic acid. Two lectins, LVL-1 and LVL-2, were purified by gel filtration on Sepharose 4B succeeded by ion-exchange chromatography on Mono-Q. Both lectins were glycoproteins composed of covalently bonded subunits of 53 kDa. The sequence of the amino-terminal region of LVL-1 was determined as Ala-Ile-Asn-Gln-Ser-Ser-Gly-Asn-Leu-(X)-Asp-Arg-Leu-Gln-Glu-Arg-Phe-(X)-Leu-Asp- His-, where X is an unidentified residue.

AB - The extract of the soft coral Lobophytum variatum agglutinated horse erythrocytes but not human ABO or rabbit erythrocytes in the presense of calcium ions. The activity was inhibited by mucin Type I from the bovine submaxillary gland and also by simple sugars such as D-ribose and N-acetylneuraminic acid. Two lectins, LVL-1 and LVL-2, were purified by gel filtration on Sepharose 4B succeeded by ion-exchange chromatography on Mono-Q. Both lectins were glycoproteins composed of covalently bonded subunits of 53 kDa. The sequence of the amino-terminal region of LVL-1 was determined as Ala-Ile-Asn-Gln-Ser-Ser-Gly-Asn-Leu-(X)-Asp-Arg-Leu-Gln-Glu-Arg-Phe-(X)-Leu-Asp- His-, where X is an unidentified residue.

KW - Agglutinin

KW - Hemagglutinin

KW - Lectin

KW - Lobophytum variatum

KW - Soft coral

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DO - 10.2331/fishsci.62.297

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JO - Fisheries Science

JF - Fisheries Science

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ER -

Purification and Characterization of the Lectins of the Soft Coral Lobophytum variatum

Abstract

Keywords

ASJC Scopus subject areas

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