Purification and characterization of small and large rubber particles from Hevea brasiliensis

Satoshi Yamashita, Makie Mizuno, Hidehiko Hayashi, Haruhiko Yamaguchi, Yukino Miyagi-Inoue, Kazuhisa Fushihara, Tanetoshi Koyama, Toru Nakayama, Seiji Takahashi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Natural rubber (NR) is synthesized by the rubber transferase (RTase) on rubber particles (RPs) in latex. Due to the heterogeneity of the RPs in latex, it is difficult to precisely characterize the RTase activity. In this study, we separated the RPs of Hevea brasiliensis with different particle size distributions, via stepwise centrifugations. Analyses of protein compositions and size distributions of NR in the RPs suggest that RPs in Hevea latex can be categorized into two distinct subclasses, the larger RPs (termed 1kRP, 2kRP, and 8kRP) and the smaller RPs (termed 20kRP and 50kRP). Precise enzymatic assays using the RPs revealed that 50kRP showed the highest RTase activity, whereas the larger RPs, which had been regarded to have quite low activity, also exhibited a comparable activity to the smaller RPs. Immunological detections of cis-prenyltransferases in the RPs showed that the abundance of these enzymes correlates with the extent of RTase activity.

Original languageEnglish
Pages (from-to)1011-1020
Number of pages10
JournalBioscience, Biotechnology and Biochemistry
Volume82
Issue number6
DOIs
Publication statusPublished - 2018 Jan 1

Keywords

  • Cis-prenyltransferase
  • Hevea brasiliensis
  • Latex
  • Natural rubber
  • Rubber particle

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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