Purification and characterization of an agglutinin of the soft coral Sinularia species

Rina Goto, Koji Muramoto, Masatoshi Yamazaki, Hisao Kamiya

    Research output: Contribution to journalArticlepeer-review

    10 Citations (Scopus)

    Abstract

    A d-galactose-specific agglutinin, named sinularian, has been isolated from the soft coral Sinularia sp. by affinity chromatography on acid-treated Sepharose 4B and by gel filtration on HPLC. Sinularian was a glycoprotein containing 11% sugar. It gave a single band corresponding to 78 kDa in SDS-PAGE, irrespective of a treatment with 2-mercaptoethanol. Sinularian agglutinated rabbit erythrocytes and murine leukemia cells but not sheep or human ABO erythrocytes. Its hemagglutinating activity was Ca++-independent. Sinularian promoted binding of macrophages to tumor cells.

    Original languageEnglish
    Pages (from-to)9-17
    Number of pages9
    JournalDevelopmental and Comparative Immunology
    Volume16
    Issue number1
    DOIs
    Publication statusPublished - 1992

    Keywords

    • Agglutinin
    • Sinularia sp
    • Soft coral

    ASJC Scopus subject areas

    • Immunology
    • Developmental Biology

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