Purification and characterization of an agglutinin of the soft coral Sinularia species

Rina Goto, Koji Muramoto, Masatoshi Yamazaki, Hisao Kamiya

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11 Citations (Scopus)

Abstract

A d-galactose-specific agglutinin, named sinularian, has been isolated from the soft coral Sinularia sp. by affinity chromatography on acid-treated Sepharose 4B and by gel filtration on HPLC. Sinularian was a glycoprotein containing 11% sugar. It gave a single band corresponding to 78 kDa in SDS-PAGE, irrespective of a treatment with 2-mercaptoethanol. Sinularian agglutinated rabbit erythrocytes and murine leukemia cells but not sheep or human ABO erythrocytes. Its hemagglutinating activity was Ca++-independent. Sinularian promoted binding of macrophages to tumor cells.

Original languageEnglish
Pages (from-to)9-17
Number of pages9
JournalDevelopmental and Comparative Immunology
Volume16
Issue number1
DOIs
Publication statusPublished - 1992
Externally publishedYes

Keywords

  • Agglutinin
  • Sinularia sp
  • Soft coral

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

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