Purification and Characterization of a Cytolytic Protein from Purple Fluid of the Sea Hare, Dolabella auricularia

Masatoshi Yamazaki, Shigeru Tansho, Jun Kisugi, Koji Muramoto, Hisao Kamiya

    Research output: Contribution to journalArticlepeer-review

    26 Citations (Scopus)

    Abstract

    A novel cytolytic factor, dolabellanin P, was purified to apparent homogeneity from the purple fluid of the sea hare, Dolabella auricularia. Purified dolabellanin P is a single polypeptide of 60 kDa. The amino acid composition and the N-terminus of the factor were also determined. This factor nonspecifically lysed all the cells tested at 50—200 ng protein/ml. Dolabellanin P caused complete cytolysis within 2h. The factor is distinct from antineoplastic glycoproteins previously isolated from eggs (aplysianin E) or albumen gland (aplysianin A) of Aplysia kurodai in terms of certain cytolytic properties. These results suggest that dolabellanin P, found in the sea hare, a marine invertebrate, is a new cytolytic factor.

    Original languageEnglish
    Pages (from-to)2179-2182
    Number of pages4
    JournalChemical and Pharmaceutical Bulletin
    Volume37
    Issue number8
    DOIs
    Publication statusPublished - 1989

    Keywords

    • Dolabella auricularia
    • Keywords cytolytic factor
    • dolabellanin P
    • sea hare
    • tumor

    ASJC Scopus subject areas

    • Chemistry(all)
    • Drug Discovery

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