Abstract
6-Phospho-β-galactosidase from Lactobacillus gasseri JCM 1031 was purified from lactobacilli to homogeneity, about 118-fold, with 0.5% recovery by several chromatographies. The molecular mass and isoelectric point of the purified enzyme were 58 kDa and pl 5.5, respectively. The K m and V max for o-nitrophenyl-β-1)-ga- lactopyranoside-6-phosphate were 0.8 mm and 116.4 μmol/min/mg, respectively. Reducing agent, Fe2 + ion, and EDTA activated but PCMB, Zn2 + and Hg2 + ions strongly inhibited the enzymatic activity.
| Original language | English |
|---|---|
| Pages (from-to) | 139-141 |
| Number of pages | 3 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 60 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1996 Jan |
| Externally published | Yes |
Keywords
- 6-phospho-β-galactosidase
- Lactohacillus xassai
- Purification
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry