Purification and Characterization of γ-Hexachlorocyclohexane (γ-HCH) Dehydrochlorinase (LinA) from Pseudomonas paucimobilis

Yuji Nagata, Takashi Hatta, Ryozo Imai, Kazuhide Kimbara, Masao Fukuda, Keiji Yano, Masamichi Takagi

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)

Abstract

The linA gene from Pseudomonas paucimobilis was highly expressed in Escherichia coli, and the linA product (LinA), named γ-HCH dehydrochlorinase, was purified to homogeneity. LinA released three chloride ions per one molecule of γ-HCH. Degradation assay of halogenated compounds by purified LinA showed that the substrate specificity of LinA is very narrow.

Original languageEnglish
Pages (from-to)1582-1583
Number of pages2
JournalBioscience, Biotechnology and Biochemistry
Volume57
Issue number9
DOIs
Publication statusPublished - 1993
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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