Purification and cDNA cloning of a wound inducible glucosyltransferase active toward 12-hydroxy jasmonic acid

Yoshiya Seto, Shigeki Hamada, Hideyuki Matsuura, Mana Matsushige, Chizuru Satou, Kosaku Takahashi, Chikara Masuta, Hiroyuki Ito, Hirokazu Matsui, Kensuke Nabeta

    Research output: Contribution to journalArticlepeer-review

    20 Citations (Scopus)

    Abstract

    Tuberonic acid (12-hydroxy epi-jasmonic acid, TA) and its glucoside (TAG) were isolated from potato leaflets (Solanum tuberosum L.) and shown to have tuber-inducing properties. The metabolism of jasmonic acid (JA) to TAG in plant leaflets, and translocation of the resulting TAG to the distal parts, was demonstrated in a previous study. It is thought that TAG generated from JA transmits a signal from the damaged parts to the undamaged parts by this mechanism. In this report, the metabolism of TA in higher plants was demonstrated using [12-3H]TA, and a glucosyltransferase active toward TA was purified from the rice cell cultures. The purified protein was shown to be a putative salicylic acid (SA) glucosyltransferase (OsSGT) by MALDI-TOF-MS analysis. Recombinant OsSGT obtained by overexpression in Escherichia coli was active not only toward TA but also toward SA. The OsSGT characterized in this research was not specific, but this is the first report of a glucosyltransferase active toward TA. mRNA expressional analysis of OsSGT and quantification of TA, TAG, SA and SAG after mechanical wounding indicated that OsSGT is involved in the wounding response. These results demonstrated a crucial role for TAG not only in potato tuber formation, but also in the stress response in plants and that the SA glucosyltransferase can work for TA glucosylation.

    Original languageEnglish
    Pages (from-to)370-379
    Number of pages10
    JournalPhytochemistry
    Volume70
    Issue number3
    DOIs
    Publication statusPublished - 2009 Feb

    Keywords

    • Albizzia julibrissin
    • Arabidopsis thaliana
    • Cruciferae
    • Glycine max
    • Gramineae
    • Jasmonic acid
    • Lamidaceae
    • Leguminosae
    • Nicotiana tabacum
    • Oryza sativa
    • Salicylic acid glucosyltransferase
    • Salvia officinalis
    • Solanaceae
    • Solanum lycopersicum
    • Solanum tuberosum
    • Tuberonic acid
    • Tuberonic acid glucoside
    • Tuberonic acid glucosyltransferase
    • Wounding stress
    • Zea mays

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Plant Science
    • Horticulture

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