Pump-probe time-resolved serial femtosecond crystallography at SACLA: Current status and data collection strategies

Eriko Nango, Minoru Kubo, Kensuke Tono, So Iwata

Research output: Contribution to journalReview articlepeer-review

5 Citations (Scopus)

Abstract

Structural information on protein dynamics is a critical factor in fully understanding the protein functions. Pump-probe time-resolved serial femtosecond crystallography (TR-SFX) is a recently established technique for visualizing the structural changes or reactions in proteins that are at work with high spatial and temporal resolution. In the pump-probe method, protein microcrystals are continuously delivered from an injector and exposed to an X-ray free-electron laser (XFEL) pulse after a trigger to initiate a reaction, such as light, chemicals, temperature, and electric field, which affords the structural snapshots of intermediates that occur in the protein. We are in the process of developing the device and techniques for pump-probe TR-SFX while using XFEL produced at SPring-8 Angstrom Compact Free-Electron Laser (SACLA). In this paper, we described our current development details and data collection strategies for the optical pump X-ray probe TR-SFX experiment at SACLA and then reported the techniques of in crystallo TR spectroscopy, which is useful in clarifying the nature of reaction that takes place in crystals in advance.

Original languageEnglish
Article number5505
JournalApplied Sciences (Switzerland)
Volume9
Issue number24
DOIs
Publication statusPublished - 2019 Dec 1
Externally publishedYes

Keywords

  • Microcrystals
  • Pump-probe experiment
  • Time-resolved crystallography
  • X-ray free-electron laser

ASJC Scopus subject areas

  • Materials Science(all)
  • Instrumentation
  • Engineering(all)
  • Process Chemistry and Technology
  • Computer Science Applications
  • Fluid Flow and Transfer Processes

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