Pull-down method to access the cell surface receptor for Toxoplasma gondii

Haiyan Gong, Kyousuke Kobayashi, Tatsuki Sugi, Hitoshi Takemae, Taisuke Horimoto, Xuenan Xuan, Hiroomi Akashi, Kentaro Kato

Research output: Contribution to journalArticlepeer-review

Abstract

Protein X, which is expressed on the surface of Toxoplasma and is thought to interact with host molecules, was expressed as a GST recombinant protein, conjugated to sepharose 4B, and used to pull down biotin-labeled 293T cells. The product was analysis by 2D-PAGE and Western blotting. Mass spectrometry revealed the reacted spots from the gel to be heat shock proteins.

Original languageEnglish
Pages (from-to)514-515
Number of pages2
JournalParasitology International
Volume65
Issue number5
DOIs
Publication statusPublished - 2016 Oct 1
Externally publishedYes

Keywords

  • Biotin
  • Mass spectrometry (MS)
  • Pull-down method
  • Streptavidin
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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