PTHrP induces notch signaling in periodontal ligament cells

A. Nakao, H. Kajiya, H. Fukushima, A. Fukushima, H. Anan, S. Ozeki, K. Okabe

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)


Periodontal ligament (PDL) cells are known to play important roles in tooth eruption and alveolar bone metabolism. We previously reported that PTHrP increases RANKL expression in human PDL cells, suggesting that it promotes odontoclastic root resorption during tooth eruption. While it is known that Notch-related genes play a key role during bone development, the role of the Notch signaling pathway in PDL cells during tooth and bone resorption is less clear. We hypothesized that PTHrP induces a Notch ligand in PDL cells and thereby regulates osteo- and odontoclastogenesis. We found that PTHrP increased Notch1 ligand Jagged1 expression in human PDL cells in a dose- and time-dependent manner. PTHrP-induced Jagged1 up-regulation was mediated by PKA activation, but not by PKC. Jagged1 also promoted RANKL-induced osteoclas- togenesis. These results demonstrate that PTHrP induces Jagged1 expression in PDL cells, leading to osteo- and odontoclastogenesis, and thus likely promoting tooth and alveolar bone resorption. Abbreviations: PTHrP, parathyroid-hormone- related protein; PTH/PTHrP type 1 receptor, PTH1R; RANKL, receptor activator of NF-κB ligand; OPG, osteoprotegerin; PDL, periodontal ligament; TRAP, tartrate-resistant acid phosphatase; PKA, protein kinase A; PKC, protein kinase C.

Original languageEnglish
Pages (from-to)551-556
Number of pages6
JournalJournal of dental research
Issue number6
Publication statusPublished - 2009 Jun 1


  • Jagged1
  • PKA
  • Periodontal ligament
  • Pthrp

ASJC Scopus subject areas

  • Dentistry(all)


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