Prototyping a versatile two-layer multi-channel microfluidic device for direct-contact cell-vessel co-culture

Li Jiun Chen, Bibek Raut, Nobuhiro Nagai, Toshiaki Abe, Hirokazu Kaji

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Microfluidic devices are gaining increasing popularity due to their wide applications in various research areas. Herein, we propose a two-layer multi-channel microfluidic device allowing for direct-contact cell-vessel co-culture. Using the device, we built a co-culture model of the outer blood-retina barrier (oBRB), mimicking the in vivo retinal pigment epithelial cells-Bruch membrane-fenestrated choroids. To demonstrate the versatility of the design, we further modified the device by inserting platinum electrodes for trans-epithelial electrical resistance (TEER) measurement, demonstrating the feasibility of on-chip assessment of the epithelial barrier integrity. Our proposed design allows for direct-contact co-culture of cell-cell or cell-vessel, modifiable for real-time evaluation of the state of the epithelial monolayers.

Original languageEnglish
Article number79
JournalMicromachines
Volume11
Issue number1
DOIs
Publication statusPublished - 2020 Jan 1

Keywords

  • Microfabrication
  • Microfluidics
  • Multi-culture
  • Organ-on-a-chip
  • Trans-epithelial electrical resistance

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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