Proton NMR Study of the Trimannosyl Unit in a Pentaantennary N‐Linked Decasaccharide Structure: Complete Assignment of the Proton Resonances and Conformational Characterization

Tomohiko Taguchi, Ken Kitajima, Yutaka Muto, Shigeyuki Yokoyama, Sadako Inoue, Yasuo Inoue

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The chemical shifts of all the ring protons of the three Man residues in a pentaantennary glycan chain have been unambiguously assigned by two‐dimensional proton nuclear magnetic resonance (1H‐NMR) spectroscopic methods. The study, using chemical shift and J values on the conformation of the trimannosyl unit, revealed that the rotamer about the C5‐C6 bond of the α1→6 linkage in the sequence of Manα1→6Manβ1→ is predominantly confined to a gauche‐gauche rotamer (ω= 180°, ω= O6‐C6‐C5‐H5) and not to a gauche‐trans rotamer (ω=–60°). We do not know of any previous demonstration that the dihedral angle ω (O6‐C6‐C5‐H5) in Manα1→6Manβ1→ is preferentially 180° in complex‐type N‐linked glycans having no bisecting GlcNAc residue.

Original languageEnglish
Pages (from-to)822-829
Number of pages8
JournalEuropean Journal of Biochemistry
Volume228
Issue number3
DOIs
Publication statusPublished - 1995 Mar
Externally publishedYes

Keywords

  • H‐NMR
  • Pentaantennary glycan
  • gauche‐gauche rotamer
  • hyosophorin

ASJC Scopus subject areas

  • Biochemistry

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