Abstract
The chemical shifts of all the ring protons of the three Man residues in a pentaantennary glycan chain have been unambiguously assigned by two‐dimensional proton nuclear magnetic resonance (1H‐NMR) spectroscopic methods. The study, using chemical shift and J values on the conformation of the trimannosyl unit, revealed that the rotamer about the C5‐C6 bond of the α1→6 linkage in the sequence of Manα1→6Manβ1→ is predominantly confined to a gauche‐gauche rotamer (ω= 180°, ω= O6‐C6‐C5‐H5) and not to a gauche‐trans rotamer (ω=–60°). We do not know of any previous demonstration that the dihedral angle ω (O6‐C6‐C5‐H5) in Manα1→6Manβ1→ is preferentially 180° in complex‐type N‐linked glycans having no bisecting GlcNAc residue.
Original language | English |
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Pages (from-to) | 822-829 |
Number of pages | 8 |
Journal | European Journal of Biochemistry |
Volume | 228 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1995 Mar |
Externally published | Yes |
Keywords
- H‐NMR
- Pentaantennary glycan
- gauche‐gauche rotamer
- hyosophorin
ASJC Scopus subject areas
- Biochemistry