Protocols for isolation and evaluation of muse cells

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

This chapter provides the detailed method for isolation of Muse cells and evaluation of their pluripotency. The basic population of Muse cells is cultured mesenchymal stem cells such as bone marrow-mesenchymal stem cells, fibroblasts, and adipose-derived stem cells. The detailed method for handling mesenchymal stem cells is also provided in this protocol.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages69-101
Number of pages33
DOIs
Publication statusPublished - 2018

Publication series

NameAdvances in Experimental Medicine and Biology
Volume1103
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

Keywords

  • Adipose-derived stem cells
  • Anti-SSEA-3 antibody
  • Cell sorting
  • Clusters
  • FACS
  • Fibroblasts
  • MSCs
  • Serum lot check

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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