TY - JOUR
T1 - Proteomic analysis of early melanosomes
T2 - Identification of novel melanosomal proteins
AU - Basrur, Venkatesha
AU - Yang, Feng
AU - Kushimoto, Tsuneto
AU - Higashimoto, Youichiro
AU - Yasumoto, Ken ichi
AU - Valencia, Julio
AU - Muller, Jacqueline
AU - Vieira, Wilfred D.
AU - Watabe, Hidenori
AU - Shabanowitz, Jeffrey
AU - Hearing, Vincent J.
AU - Hunt, Donald F.
AU - Appella, Ettore
PY - 2003
Y1 - 2003
N2 - Melanin is a heterogeneous biopolymer produced only by specific cells termed melanocytes, which synthesize and deposit the pigment in specialized membrane-bound organelles known as melanosomes. Although melanosomes have been suspected of being closely related to lysosomes and platelets, the total number of melanosomal proteins is still unknown. Thus far, six melanosome-specific proteins have been identified, and the challenge is to characterize the complete proteome of the melanosome to further understand its mechanism of biogenesis. In this report, we used mass spectrometry and subcellular fractionation to identify protein components of early melanosomes. Using this approach, we have identified all 6 of the known melanosome-specific proteins, 56 proteins that are shared with other organelles, and confirmed the presence of 6 novel melanosomal proteins using western blotting and by immunohistochemistry.
AB - Melanin is a heterogeneous biopolymer produced only by specific cells termed melanocytes, which synthesize and deposit the pigment in specialized membrane-bound organelles known as melanosomes. Although melanosomes have been suspected of being closely related to lysosomes and platelets, the total number of melanosomal proteins is still unknown. Thus far, six melanosome-specific proteins have been identified, and the challenge is to characterize the complete proteome of the melanosome to further understand its mechanism of biogenesis. In this report, we used mass spectrometry and subcellular fractionation to identify protein components of early melanosomes. Using this approach, we have identified all 6 of the known melanosome-specific proteins, 56 proteins that are shared with other organelles, and confirmed the presence of 6 novel melanosomal proteins using western blotting and by immunohistochemistry.
KW - Melanoma
KW - Melanosome
KW - Proteomics
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U2 - 10.1021/pr025562r
DO - 10.1021/pr025562r
M3 - Article
C2 - 12643545
AN - SCOPUS:12244267707
VL - 2
SP - 69
EP - 79
JO - Journal of Proteome Research
JF - Journal of Proteome Research
SN - 1535-3893
IS - 1
ER -