Proteolytic Enzyme Sensors Using an Ion-Sensitive Field Effect Transistor

Hongda Fu; Jun ichi Anzai; Tetsuo Osa; Tadayuki Matsuo

doi:10.1248/cpb.36.1190

Proteolytic Enzyme Sensors Using an Ion-Sensitive Field Effect Transistor

Hongda Fu, Jun ichi Anzai, Tetsuo Osa, Tadayuki Matsuo

PHA - Molecular Pharmaceutical Science

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Two kinds of enzyme sensors were fabricated by chemically immobilizing an enzyme on the gate surface of an ion-sensitive field effect transistor (ISFET). One is a trypsin-membrane/ ISFET sensor, which can measure concentrations of JV-benzoyl-L-arginine ethyl ester in the range from 5 x 10^-4to 5 x 10^–2m. The other is an a-chymotrypsin-membrane/ISFET sensor, which can measure concentrations of N-acetyl-L-tyrosine ethyl ester solution in the range of 5xl0^-4—5 x 10^–3m. The response of the sensors was affected markedly by pH and the concentration of the working buffer.

Original language	English
Pages (from-to)	1190-1193
Number of pages	4
Journal	Chemical and Pharmaceutical Bulletin
Volume	36
Issue number	3
DOIs	https://doi.org/10.1248/cpb.36.1190
Publication status	Published - 1988

Keywords

a-chymotrypsin membrane
enzyme sensor
ion-sensitive field effect transistor
proteolytic enzyme sensor
trypsin membrane

ASJC Scopus subject areas

Chemistry(all)
Drug Discovery

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title = "Proteolytic Enzyme Sensors Using an Ion-Sensitive Field Effect Transistor",

abstract = "Two kinds of enzyme sensors were fabricated by chemically immobilizing an enzyme on the gate surface of an ion-sensitive field effect transistor (ISFET). One is a trypsin-membrane/ ISFET sensor, which can measure concentrations of JV-benzoyl-L-arginine ethyl ester in the range from 5 x 10-4to 5 x 10–2m. The other is an a-chymotrypsin-membrane/ISFET sensor, which can measure concentrations of N-acetyl-L-tyrosine ethyl ester solution in the range of 5xl0-4—5 x 10–3m. The response of the sensors was affected markedly by pH and the concentration of the working buffer.",

keywords = "a-chymotrypsin membrane, enzyme sensor, ion-sensitive field effect transistor, proteolytic enzyme sensor, trypsin membrane",

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T1 - Proteolytic Enzyme Sensors Using an Ion-Sensitive Field Effect Transistor

AU - Fu, Hongda

AU - Anzai, Jun ichi

AU - Osa, Tetsuo

AU - Matsuo, Tadayuki

PY - 1988

Y1 - 1988

N2 - Two kinds of enzyme sensors were fabricated by chemically immobilizing an enzyme on the gate surface of an ion-sensitive field effect transistor (ISFET). One is a trypsin-membrane/ ISFET sensor, which can measure concentrations of JV-benzoyl-L-arginine ethyl ester in the range from 5 x 10-4to 5 x 10–2m. The other is an a-chymotrypsin-membrane/ISFET sensor, which can measure concentrations of N-acetyl-L-tyrosine ethyl ester solution in the range of 5xl0-4—5 x 10–3m. The response of the sensors was affected markedly by pH and the concentration of the working buffer.

AB - Two kinds of enzyme sensors were fabricated by chemically immobilizing an enzyme on the gate surface of an ion-sensitive field effect transistor (ISFET). One is a trypsin-membrane/ ISFET sensor, which can measure concentrations of JV-benzoyl-L-arginine ethyl ester in the range from 5 x 10-4to 5 x 10–2m. The other is an a-chymotrypsin-membrane/ISFET sensor, which can measure concentrations of N-acetyl-L-tyrosine ethyl ester solution in the range of 5xl0-4—5 x 10–3m. The response of the sensors was affected markedly by pH and the concentration of the working buffer.

KW - a-chymotrypsin membrane

KW - enzyme sensor

KW - ion-sensitive field effect transistor

KW - proteolytic enzyme sensor

KW - trypsin membrane

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JF - Chemical and Pharmaceutical Bulletin

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Proteolytic Enzyme Sensors Using an Ion-Sensitive Field Effect Transistor

Abstract

Keywords

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