Proteolytic Enzyme Sensors Using an Ion-Sensitive Field Effect Transistor

Hongda Fu, Junichi Anzai, Tetsuo Osa, Tadayuki Matsuo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Two kinds of enzyme sensors were fabricated by chemically immobilizing an enzyme on the gate surface of an ion-sensitive field effect transistor (ISFET). One is a trypsin-membrane/ ISFET sensor, which can measure concentrations of JV-benzoyl-L-arginine ethyl ester in the range from 5 x 10-4to 5 x 10–2m. The other is an a-chymotrypsin-membrane/ISFET sensor, which can measure concentrations of N-acetyl-L-tyrosine ethyl ester solution in the range of 5xl0-4—5 x 10–3m. The response of the sensors was affected markedly by pH and the concentration of the working buffer.

Original languageEnglish
Pages (from-to)1190-1193
Number of pages4
JournalChemical and Pharmaceutical Bulletin
Volume36
Issue number3
DOIs
Publication statusPublished - 1988 Jan 1

Keywords

  • a-chymotrypsin membrane
  • enzyme sensor
  • ion-sensitive field effect transistor
  • proteolytic enzyme sensor
  • trypsin membrane

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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