Abstract
Two kinds of enzyme sensors were fabricated by chemically immobilizing an enzyme on the gate surface of an ion-sensitive field effect transistor (ISFET). One is a trypsin-membrane/ ISFET sensor, which can measure concentrations of JV-benzoyl-L-arginine ethyl ester in the range from 5 x 10-4to 5 x 10–2m. The other is an a-chymotrypsin-membrane/ISFET sensor, which can measure concentrations of N-acetyl-L-tyrosine ethyl ester solution in the range of 5xl0-4—5 x 10–3m. The response of the sensors was affected markedly by pH and the concentration of the working buffer.
| Original language | English |
|---|---|
| Pages (from-to) | 1190-1193 |
| Number of pages | 4 |
| Journal | Chemical and Pharmaceutical Bulletin |
| Volume | 36 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1988 Jan 1 |
Keywords
- a-chymotrypsin membrane
- enzyme sensor
- ion-sensitive field effect transistor
- proteolytic enzyme sensor
- trypsin membrane
ASJC Scopus subject areas
- Chemistry(all)
- Drug Discovery
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Fu, H., Anzai, J., Osa, T., & Matsuo, T. (1988). Proteolytic Enzyme Sensors Using an Ion-Sensitive Field Effect Transistor. Chemical and Pharmaceutical Bulletin, 36(3), 1190-1193. https://doi.org/10.1248/cpb.36.1190