Abstract
Two kinds of enzyme sensors were fabricated by chemically immobilizing an enzyme on the gate surface of an ion-sensitive field effect transistor (ISFET). One is a trypsin-membrane/ ISFET sensor, which can measure concentrations of JV-benzoyl-L-arginine ethyl ester in the range from 5 x 10-4to 5 x 10–2m. The other is an a-chymotrypsin-membrane/ISFET sensor, which can measure concentrations of N-acetyl-L-tyrosine ethyl ester solution in the range of 5xl0-4—5 x 10–3m. The response of the sensors was affected markedly by pH and the concentration of the working buffer.
Original language | English |
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Pages (from-to) | 1190-1193 |
Number of pages | 4 |
Journal | Chemical and Pharmaceutical Bulletin |
Volume | 36 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1988 |
Keywords
- a-chymotrypsin membrane
- enzyme sensor
- ion-sensitive field effect transistor
- proteolytic enzyme sensor
- trypsin membrane
ASJC Scopus subject areas
- Chemistry(all)
- Drug Discovery