Protein sensors based on the potentiometric photoresponse of polymer membranes doped with photochromic spiropyran

Jun ichi Anzai; Kenji Sakamura; Yasushi Hasebe; Tetsuo Osa

doi:10.1016/0003-2670(93)85013-A

Protein sensors based on the potentiometric photoresponse of polymer membranes doped with photochromic spiropyran

Jun ichi Anzai, Kenji Sakamura, Yasushi Hasebe, Tetsuo Osa

PHA - Molecular Pharmaceutical Science

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The potentiometric photoresponse of plasticized polymer [carboxylated poly(vinyl chloride)] membranes doped with a spirobenzopyran derivative was modulated significantly by specific adsorption of proteins on the membrane surface. The magnitude of the photoinduced potential of the membranes was decreased in the presence of 1-15 μg ml^-1 of anti-DNP or 0.1-1.5 μg ml^-1 of avidin in solution. The possible use of the membranes as protein sensor materials is discussed in terms of response time, selectivity and re-usability.

Original language	English
Pages (from-to)	543-548
Number of pages	6
Journal	Analytica Chimica Acta
Volume	281
Issue number	3
DOIs	https://doi.org/10.1016/0003-2670(93)85013-A
Publication status	Published - 1993 Sep 24

Keywords

Biosensors
Membrane electrodes
Potentiometry
Proteins
Spirobenzopyran

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy

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10.1016/0003-2670(93)85013-A

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abstract = "The potentiometric photoresponse of plasticized polymer [carboxylated poly(vinyl chloride)] membranes doped with a spirobenzopyran derivative was modulated significantly by specific adsorption of proteins on the membrane surface. The magnitude of the photoinduced potential of the membranes was decreased in the presence of 1-15 μg ml-1 of anti-DNP or 0.1-1.5 μg ml-1 of avidin in solution. The possible use of the membranes as protein sensor materials is discussed in terms of response time, selectivity and re-usability.",

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T1 - Protein sensors based on the potentiometric photoresponse of polymer membranes doped with photochromic spiropyran

AU - Anzai, Jun ichi

AU - Sakamura, Kenji

AU - Hasebe, Yasushi

AU - Osa, Tetsuo

PY - 1993/9/24

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N2 - The potentiometric photoresponse of plasticized polymer [carboxylated poly(vinyl chloride)] membranes doped with a spirobenzopyran derivative was modulated significantly by specific adsorption of proteins on the membrane surface. The magnitude of the photoinduced potential of the membranes was decreased in the presence of 1-15 μg ml-1 of anti-DNP or 0.1-1.5 μg ml-1 of avidin in solution. The possible use of the membranes as protein sensor materials is discussed in terms of response time, selectivity and re-usability.

AB - The potentiometric photoresponse of plasticized polymer [carboxylated poly(vinyl chloride)] membranes doped with a spirobenzopyran derivative was modulated significantly by specific adsorption of proteins on the membrane surface. The magnitude of the photoinduced potential of the membranes was decreased in the presence of 1-15 μg ml-1 of anti-DNP or 0.1-1.5 μg ml-1 of avidin in solution. The possible use of the membranes as protein sensor materials is discussed in terms of response time, selectivity and re-usability.

KW - Biosensors

KW - Membrane electrodes

KW - Potentiometry

KW - Proteins

KW - Spirobenzopyran

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