Protein sensors based on the potentiometric photoresponse of polymer membranes doped with photochromic spiropyran

Jun ichi Anzai, Kenji Sakamura, Yasushi Hasebe, Tetsuo Osa

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The potentiometric photoresponse of plasticized polymer [carboxylated poly(vinyl chloride)] membranes doped with a spirobenzopyran derivative was modulated significantly by specific adsorption of proteins on the membrane surface. The magnitude of the photoinduced potential of the membranes was decreased in the presence of 1-15 μg ml-1 of anti-DNP or 0.1-1.5 μg ml-1 of avidin in solution. The possible use of the membranes as protein sensor materials is discussed in terms of response time, selectivity and re-usability.

Original languageEnglish
Pages (from-to)543-548
Number of pages6
JournalAnalytica Chimica Acta
Volume281
Issue number3
DOIs
Publication statusPublished - 1993 Sep 24

Keywords

  • Biosensors
  • Membrane electrodes
  • Potentiometry
  • Proteins
  • Spirobenzopyran

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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