Floral organs (tepal, stamen, style, including stigma, and ovary) from immature and mature (1 day prior to anthesis) flower buds of pin and thrum morphs of Averrhoa carambola were subjected to one and two-dimensional IEF/SDS-PAGE gel electrophoresis and visualized by silver staining. One-dimensional gel electrophoresis of organ extracts from mature floral buds showed a number of protein bands common to all organs in both pin and thrum morphs. In the stamen and style, these bands differed in intensity between the two morphs. Under two-dimensional gels, the differences in protein profiles between the two morphs were more distinct in these organs. When compared with polypeptide spots from leaflets, a total of 14 floral organ-specific polypeptides was detected, the majority appearing in the stamen, followed by the style but none in the ovary. In the stamen, most of these polypeptides were detected in both the pin and thrum morphs. However, in the style, a 72-kDa polypeptide was detected exclusively in the pin morph, and this was also the most abundant floral organ-specific polypeptide. Floral organ-specific polypeptides of 45 kDa (detected in stamens of the thrum morph) and 70 kDa (detected in stamen and style of both morphs) were found to bind concanavalin A.
- Averrhoa carambola
- Electrophoresis Carambola/star-fruit
- Heterostyly Flower organ-specific proteins
ASJC Scopus subject areas
- Plant Science
- Cell Biology